Morphological characterisation of yeast colony growth on solid media using image processing

作     者：Robinson, A Sadr-kazemi, N Dickason, G Harrison, STL 

作者机构：Univ Cape Town ZA-7701 Cape Town South Africa 

出 版 物：《BIOTECHNOLOGY TECHNIQUES》 (生物技术工艺)

年 卷 期：1998年第12卷第10期

页      面：763-767页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0836[工学-生物工程] 

主　　题：image processing Watershed algorithm morphology growth rate 

摘      要：To rapidly determine the effect of environmental factors on yeast growth, a cell counting and colony sizing image analysis method was developed to characterise colony growth on solid media. A digitised microscopic image of the yeast was analysed using the Watershed algorithm for cell number determination and a morphological edge detection for colony size determination. The influence of temperature and physiological stress on yeast growth was then investigated over 12.5 h and data extracted by the image analysis method.