Chloromethane metabolism by <i>Methylobacterium</i> sp. strain CM4

作     者：Vannelli, T Studer, A Kertesz, M Leisinger, T 

作者机构：ETH Zurich Inst Mikrobiol CH-8092 Zurich Switzerland 

出 版 物：《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 (Appl. Environ. Microbiol.)

年 卷 期：1998年第64卷第5期

页      面：1933-1936页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

摘      要：Methylobacterium sp. strain CM4 metabolized chloromethane quantitatively with a molar yield of 2.8 g of whole-cell protein/mol of C. This value was similar to that observed after growth with methanol (2.9 g of protein/mol of C) and about three times larger than the yield with formate (0.94 g of protein/mol of C). Chloromethane dehalogenation activity was inducible. MiniTn5 transposon insertion mutants with altered growth characteristics with chloromethane and other C-1 compounds were isolated and characterized. Nine of these were unable to grow with chloromethane but were able to grow with methanol, methylamine, or formate. Seventy-three transposon mutants that were defective in the utilization of either methanol, methylamine, methanol plus methylamine, or formate could still grow with chloromethane. Based on the protein yield data and the properties of the transposon mutants, we propose a pathway for chloromethane metabolism that depends on methyltransferase and dehydrogenase activities.