The organization of serotonin-, dopamine-, and FMRFamide-containing neuronal elements and their possible role in the regulation of spontaneous contraction of the gastrointestinal tract in the snail <i>Helix pomatia</i>

作     者：Hernádi, L Erdélyi, L Hiripi, L Elekes, K 

作者机构：Hungarian Acad Sci Balaton Limnol Res Inst H-8237 Tihany Hungary Attila Jozsef Univ Dept Comparat Anim Physiol Szeged Hungary 

出 版 物：《JOURNAL OF NEUROCYTOLOGY》 (脑细胞生物学杂志)

年 卷 期：1998年第27卷第10期

页      面：761-775页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071003[理学-生理学] 

基　　金：Volkswagen-Foundation Hungarian Scientific Research Fund, OTKA, (16015) 

主　　题：多巴胺/分析 多巴胺/药理学 食管/神经支配 食管/生理学 FMRF酰胺/分析 FMRF酰胺/药理学 胃肠活动/药物作用 胃肠活动/生理学 砂囊/神经支配 砂囊/生理学 苹果蜗牛属/生理学 肠/神经支配 肠/生理学 肌收缩/药物作用 肌收缩/生理学 神经元/化学 神经肽类/分析 神经肽类/药理学 血清素/分析 血清素/药理学 动物 

摘      要：The distribution of serotonin-, tyrosine hydroxylase-, and FMRFamide-immunoreactive neuronal elements, as well as the concentrations of serotonin and dopamine in the different parts of the gastrointestinal tract, were studied in the snail Helix pomatia. The sensitivity of the spontaneous contractions of the alimentary tract to serotonin, dopamine, and FMRFamide was also tested. Serotonin-, tyrosine hydroxylase-, and FMRFamide-immunoreactive elements could be demonstrated in each part of the gastrointestinal tract, but they showed different innervation patterns. Serotonin- and tyrosine hydroxylase-immunoreactive elements were dominant in the submucosal layer, whereas FMRFamide-immunoreactive elements were dominant in both the mucosal and submucosal layers. Tyrosine hydroxylase-immunoreactive elements were confined to the longitudinal muscle trabeculae of submucosa, whereas serotonin-immunoreactive elements were distributed throughout the submucosal layer. No serotonin-immunoreactive cell bodies, but only fibers, could be detected in the gastrointestinal tract, and therefore they represent extrinsic elements. Tyrosine hydroxylase- and FMRFamide-immunoreactive cell bodies represent intrinsic elements of the tract. The occurrence and density of the serotonin- and tyrosine hydroxylase-immunoreactive elements showed significant differences in the different parts of the alimentary tract, in accordance with HPLC assays, which revealed a significant frontocaudal decrease in both the serotonin (from 2.11 to 1.21 pM/mg) and dopamine (from 3.28 to 0.52 pM/mg) contents of the different parts of the alimentary tract. Dopamine at 10(-5) M concentration proved to be effective only on the longitudinal muscles by increasing the tone and frequency of contractions, but was ineffective on the circular muscles. Serotonin affected both the longitudinal and circular muscles. Serotonin at 10(-5) M concentration decreased the tone and increased the frequency of low-amplitude contractions of