INVOLVEMENT OF PLASTOQUINONE AND LIPIDS IN ELECTRON-TRANSPORT REACTIONS MEDIATED BY THE CYTOCHROME B6-F COMPLEX ISOLATED FROM SPINACH

作     者：CHAIN, RK 

作者机构：Division of Molecular Plant Biology University of California Berkeley CA 94720 USA 

出 版 物：《FEBS LETTERS》 (欧洲生化学会联合会快报)

年 卷 期：1985年第180卷第2期

页      面：321-325页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：National Institute of Health 

主　　题：Cytochrome b 6 -f complex Plastoquinone Lipid Reconstitution PC, phosphatidylcholine PG, phosphatidylglycerol MGDG monogalactosyldiacylglycerol DGDG, digalactosyldiacylglycerol SQDG, sulfoquinovosyldiacylglycerol TLC, thin-layer chromatography DNP-INT, 2,4-dinitrophenyl ether of iodonitrothymol, PQ, plastoquinone b 6 , cytochrome b 6 f , cytochrome f 

摘      要：The isolation of a cytochrome b6-f complex from spinach, which is depleted of plastoquinone (and lipid), is reported. The depleted complex no longer functions as a plastoquinol-plastocyanin oxidoreductase but can be reconstituted with plastoquinone and exogenous lipids. The lipid classes digalactosyldiacylglycerol, phosphatidylglycerol and phosphatidylcholine were active in reconstitution while monogalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol were not. Neither plastoquinone nor lipid alone fully reconstitutes electron transport in the depleted complex. Saturation of plastoquinol-plastocyanin oxidoreductase activity in the depleted complex occurs at 1 plastoquinone per cytochrome f.