BACTERIOCIDAL ACTION OF MITOMYCIN C

作     者：REICH, E SHATKIN, AJ TATUM, EL 

作者机构：Laboratory of Biochemical Genetics Rockefeller Institute New York N.Y. U.S.A. 

出 版 物：《BIOCHIMICA ET BIOPHYSICA ACTA》 (生物化学与生物物理学报)

年 卷 期：1961年第53卷第1期

页      面：132-+页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：F genetic factor determining sexual polarity in E. coli K12 Hfr H Hayes strain of E. coli K12 showing high frequency of recombination 

摘      要：The bacteriocidal action of Mitomycin C has been investigated with respect to its biochemical, genetic and morphological correlates. All findings are consistent with the observation that cells treated with Mitomycin lose DNA, but not RNA or protein, to the surrounding medium. Substantial proportions of DNA thymine appear in acid-soluble form, largely as the free base. Mitomycin attacks growing as well as non-growing cells. It may be used for concentrating non-lysogenic cells in lysogenic populations, but not for the preferential selection of biochemical mutants.