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内蒙古自治区呼和浩特市赛罕区大学西街235号 邮编: 010021
作者机构:UNIV AMSTERDAM ACAD MED CTR EC SLATER INST BIOCHEM RES 1105 AZ AMSTERDAM NETHERLANDS
出 版 物:《BIOCHIMICA ET BIOPHYSICA ACTA》 (Biochim. Biophys. Acta Gen. Subj.)
年 卷 期:1990年第1035卷第1期
页 面:6-11页
核心收录:
学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学]
主 题:ATPase ATPase, F 1 F 0 - permeability peroxisome
摘 要:Highly purified peroxisomal fractions from rat liver contain ATPase activity (18.8 .+-. 0.1 nmol/min per mg, n = 6). This activity is about 2% of that found in purified mitochondrial fractions. Measurement of marker enzyme activities and immunoblotting of the peroxisomal fraction with an antiserum raised against the .beta.-subunit of mitochondrial ATPase indicates that the ATPase activity in the peroxisomal fractions can not be ascribed to contamination with mitochondria or other subcellular organelles. From the sensitivity of the ATPase present in the peroxisomal fraction towards a variety of ATPase inhibitors, we conclude that it displays both V-type and F-type features and is distinguishable from both the mitochondrial F1F0-ATPase and the lysosomal V-type ATPase.