The role of neutrophil in the cerebral injury of ischemia/reperfused diabetic rats

作     者：王文安 任惠民 吕传真 

作者机构：XinhuaHospitalShanghaiSecondMedicalUniversityShanghai200092China 

出 版 物：《中国临床康复》 (Chinese Journal of Clinical Rehabilitation)

年 卷 期：2002年第6卷第13期

页      面：2020-2021页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100204[医学-神经病学] 10[医学] 

主　　题：糖尿病 脑缺血 中性白细胞 缺血再灌注损伤 

摘      要：Objective To explore the role of neutrophil in focal cerebral injury of ischemia/reperfused diabetic rats. Methods The infarction size in diabetic and non diabetic rats with ischemia for 1h/reperfusion for 24 h was evaluated by 2,3,5 triphenyl tetrazolium chloride (TTC) staining. The magnitude of cerebral neutrophil infiltration was quantified by measuring myeloperoxidase (MPO) activity of brain tissue in all rats with ischemia for 1h/ reperfused various periods by the method of enzymology. Results The infarction size in the ipsilateral hemisphere of diabetic rats were more significant than that in the normal rats (P 0.05). The activity of myeloperoxidase (MPO) in the former was also higher than that in the latter (P 0.05). Conclusions Diabetes could exacerbate inflammatory response to cerebral ischemia reperfusion and thereby aggravate cerebral injury of ischemia/reperfusion.