Micro-perforated elastomeric poly(dimethylsiloxane) mask fabricated using high-aspect-ratio micro-pillar arrays for spatially defined surface modification: an unconventional method for establishing a microarray platform

作     者：Tang, Linzhi Lee, Nae Yoon 

作者机构：Kyungwon Univ Gachon BioNano Res Inst Songnam 461701 Gyeonggi Do South Korea Kyungwon Univ Div BioNano Technol Songnam 461701 Gyeonggi Do South Korea Kyungwon Univ Coll BioNano Technol Songnam 461701 Gyeonggi Do South Korea 

出 版 物：《ANALYTICAL AND BIOANALYTICAL CHEMISTRY》 (弗雷泽纽斯分析化学杂志)

年 卷 期：2009年第394卷第4期

页      面：1227-1232页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：Korean Government (MOEHRD) [KRF-2008-531-D00002, M10755160002-07N5516-00210] Kyungwon University 

主　　题：High-aspect-ratio micro-pillar arrays Thiol-ene adhesive Micro-perforation array Elastomeric PDMS mask Selective surface modification Microarray platform 

摘      要：Herein, we introduce the fabrication of a micro-perforated elastomeric poly(dimethylsiloxane) (PDMS) mask and employ it for spatially defined surface modification. To fabricate the micro-perforated PDMS mask, high-aspect-ratio micro-pillar arrays having millimeter scale height were first fabricated via direct photopolymerization using a thiol-ene-based UV-curable adhesive. Square pillars (500 x 500 A mu m) and 200 A mu m circular pillars with 5 and 12.5 in the aspect ratios, respectively, were successfully fabricated with high pattern fidelity, reaching 2.5mm in height. Next, using the micro-pillar-array platform as a master mold, PDMS prepolymer was cast and polymerized to form an elastomeric PDMS mask having micro-perforation arrays. Alternating hydrophilic and hydrophobic surfaces were successfully obtained by oxidizing PDMS-covered Si wafer with corona discharge. Spatially defined chemical functionalities obtained by selective oxidation and subsequent silanization were clearly distinguished via colorimetric detection methods employing ninhydrin and toluidine reagents. The micro-perforated elastomeric PDMS mask enables selective modification of a surface without utilizing photoreactive chemicals and a photomask.