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文献详情 ><i>p27</i> inhibits CDK6/CCND1... 收藏

<i>p27</i> inhibits CDK6/CCND1 complex formation resulting in cell cycle arrest and inhibition of cell proliferation

p27 禁止 CDK6/CCND1 复杂形成导致房间周期拘捕和房间增长的抑制

作     者:Li, Niannian Zeng, Jie Sun, Fuze Tong, Xiaoling Meng, Gang Wu, Chunman Ding, Xin Liu, Lanlan Han, Minjin Lu, Cheng Dai, Fangyin 

作者机构:Southwest Univ Key Lab Sericultural Biol & Genet Breeding State Key Lab Silkworm Genome Biol Minist AgrColl Biotechnol Chongqing Peoples R China 

出 版 物:《CELL CYCLE》 (细胞周期)

年 卷 期:2018年第17卷第19-20期

页      面:2335-2348页

核心收录:

学科分类:0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基  金:National Natural Science Foundation of China [31472153, 31372379] Hi-Tech Research and Development 863 Program of China [2013AA102507] Funds of China Agriculture Research System [31472153, 31372379, 2013AA102507, CARS-18-ZJ0102] 

主  题:Cancer cell proliferation cell cycle p27 CDK6 CCND1 

摘      要:p27 plays critical roles in cell proliferation, differentiation, and apoptosis, which have been well studied in mammals and Drosophila. However, the mechanisms underlying p27 regulation of the cell cycle have not been thoroughly researched. In this study, Genevestigator, Kaplan-Meier Plotter, and the Human Protein Atlas databases were used to analyze the expression of p27, cell division protein kinase 6 (CDK6), and cyclin D1 (CCND1), as well as its prognostic value in different tumor tissues and corresponding normal tissues. Quantitative PCR and immunohistochemistry were used to detect the expression of p27, CDK6, and CCND1 in the tissues of cancer patients. The effects of p27, CDK6, and CCND1 on the proliferation of lung cancer cells were examined by the MTT assay, and flow cytometry was used to investigate the mechanism by which p27 affected cell proliferation. Immunofluorescence, co-immunoprecipitation, and Western blotting were used to determine if p27 interacted with CDK and CCND1 to regulate the cell cycle. The results showed that p27, CDK6, and CCND1 played different roles in tumorigenesis and development, which are in accordance with CDK6 and CCND1 in affecting the cell cycle and cell proliferation. p27 regulated the cell cycle and inhibited cell proliferation by affecting formation of the cell cycle-dependent complex CDK6/CCND1, but did not directly affect the expression of CDK6 and CCND1. Moreover, CCND1 did not regulate the cell cycle alone, but rather, functioned together with CDK6. This study provides insights into the effects of p27 on tumor formation and development, and the underlying regulatory mechanisms.

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