流式细胞仪在微型浮游植物生态学中的应用

作     者：孙书存 陆健健 张利华 

作者机构：华东师范大学河口海岸国家重点实验室上海200062 

出 版 物：《生态学杂志》 (Chinese Journal of Ecology)

年 卷 期：2000年第19卷第1期

页      面：72-78页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071004[理学-水生生物学] 

基　　金：河口海岸国家重点实验室与厦门大学海洋生态环境开发室基金资助 

主　　题：微型浮游植物 生态学 流式细胞仪 

摘      要：Flow cytometer (FCM) can be used to obtain the muti-parameters of the light scattering and fluorescence of individual cell rapidly and conveniently. It has been more and more applied in oceanographic and limnological studies. The work principles of FCM and its applications in the ecological studies of nano- and pico-phytoplankton were introduced and reviewed based on some recent publications abroad and at home in this paper. By means of FCM, sub-populations of phytoplankton can easily be classified, counted and sorted, and then further studies of the adaptive ability and ecological responses of the sub-populations can be implemented. It was concluded that FCM would have a prosperous prospect in the studies of the primary production and the microbial food web in marine ecosystem.