CDX2 is essential for human IVF early embryonic development

作     者：Wuwen Zhang Kai Li Xiufang Zhong Hua Yan Guoqing Tong 

作者机构：Department of Reproductive Center Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai 201203 China 

出 版 物：《生物化学与生物物理学报（英文版）》 (Acta Biochimica et Biophysica Sinica)

年 卷 期：2018年第50卷第12期

页      面：1274-1279页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：the grants from the National Natural Science Foundation of China (.81170571 and 81571442) 

主　　题：human embryo developmental block CDX2 embryonic development in vitro fertilization 

摘      要：Human in vitro fertilization (IVF) embryos usually have developmental block at 4-8 cell stages,in which only 30％-50％ percent of IVF embryos develop to blastocyst *** is the main cause for low efficiency and repeated treatment failure in human fertility *** factor CDX2 is one of the key genes in human early embryonic development,which decides the differentiation from blastomere to trophoblastic ***,the relationship between the developmental block of human IVF embryos and CDX2 is poorly *** this study,we showed that CDX2 is crucial for human IVF embryonic development.q-PCR analysis confirmed that the level of CDX2 in developmental block embryos was dramatically *** analysis further demonstrated that CDX2 protein level in developmental block embryos was significantly decreased compared with that in control *** also addressed whether reexpression of CDX2 could rescue developmental block defects in human IVF developmental block *** expected,these developmental block defects could be partially rescued by overexpression of CDX2 *** conclusion,these findings suggest that CDX2 plays an important role in the process of human early embryonic development.