MALDI-TOF mass spectrometry-based serotyping of <i>V-parahaemolyticus</i> isolated from the Zhejiang province of China

作     者：Li, Ping Xin, Wenwen Xia, Susu Luo, Yun Chen, Zhongwen Jin, Dazhi Gao, Shan Yang, Hao Ji, Bin Wang, Henghui Yan, Yong Kang, Lin Wang, Jinglin 

作者机构：Anhui Med Univ Grad Coll 81 Meishan Rd Hefei 230032 Anhui Peoples R China Jiaxing Ctr Dis Control & Prevent Jiaxing Key Lab Pathogen Microbiol 486 Wenqiao Rd Nanhu Dist 314050 Jiaxing Peoples R China Beijing Inst Microbiol & Epidemiol State Key Lab Pathogen & Biosecur 20 Dongdajie Beijing 100071 Peoples R China Zhejiang Prov Ctr Dis Control & Prevent Dept Microbiol 3399 Binsheng Rd Hangzhou 310000 Zhejiang Peoples R China 

出 版 物：《BMC MICROBIOLOGY》 (BMC微生物学)

年 卷 期：2018年第18卷第1期

页      面：1-10页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：Special Key Project of Biosafety Technologies for the National Major Research & Development Program of China [2016YFC1202400] National Hi-Tech Research and Development (863) Program of China [2014AA021402] 

主　　题：Vibrio parahaemolyticus MALDI-TOF MS O4:K8 Biomarkers 

摘      要：BackgroundVibrio parahaemolyticus is as an important food-borne pathogen circulating in China. Since 1996, the core serotype has become O3:K6, which has specific genetic markers. This serotype causes the majority of outbreaks worldwide. Until now, nearly 21 serotypes were considered as serovariants of O3:K6. Among these, O4:K68, O1:K25 and O1:KUT have caused pandemic outbreaks. O4:K8, a serovariant of O3:K6, has become the second most dominant serotype circulating in China after O3:K6. In this study, we report the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 146V. parahaemolyticus isolates belonging to 23 *** mass spectral analysis, isolates belonging to O4:K8 formed a distinct group among the five main pandemic groups (O3:K6, O4:K8, O4:K68, O1:K25 and O1:KUT). Two major protein peaks (m/z 4383 and 4397) were significantly different between serotype O4:K8 and the four other pandemic strains. Both of these peaks were present in 32 out of 36 O4:K8 isolates, but were absent in 105 out of 110 non-O4:K8 isolates. These peaks were also absent in all 74 pandemic serotypes (O3:K6, O4:K68, O1:K25 and O1:KUT).ConclusionOur results highlight the threat of O4:K8 forming a distinct group, which differs significantly from pandemic serotypes on the proteomic level. The use of MALDI-TOF MS has not been reported before in a study of this nature. Mass spectrum peaks at m/z 4383 and 4397 may be specific for O4:K8. However, we cannot conclude that MALDI-TOF MS can be used to serotype V. parahaemolyticus.