IDENTIFICATION OF UUG CODON AS A TRANSLATIONAL INITIATION CODON INVIVO

作     者：FILES, JG WEBER, K COULONDRE, C MILLER, JH 

作者机构：HARVARD UNIV BIOL LABS CAMBRIDGE MA 02138 USA MAX PLANCK INST BIOPHYS CHEM DEPT BIOCHEM GOTTINGEN FED REP GER UNIV GENEVA DEPT MOLEC BIOL GENEVA SWITZERLAND 

出 版 物：《JOURNAL OF MOLECULAR BIOLOGY》 (分子生物学杂志)

年 卷 期：1975年第95卷第2期

页      面：327-330页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：Swiss National 

主　　题：氨基酸序列 细菌蛋白质类/分析 细菌蛋白质类/分离和提纯 密码子 大肠杆菌/分析 基因 遗传学 微生物 突变 肽链起始 翻译 蛋白质生物合成 RNA 信使 

摘      要：The lac repressor protein was purified from an Escherichia coli strain carrying an amber mutation in the lacI gene and the tyrosine-inserting amber suppressor, Su3. Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber was derived from a UUG codon at this point in the message. This establishes UUG as an initiation codon in vivo , since it has been previously shown that translational reinitiation can occur at position 62.