Recursive trace line method for detecting myelinated bundles: A comparison study with pyramidal cell arrays

作     者：Casanova, Manuel F. Konkachbaev, Anouar I. Switala, Andrew E. Elmaghraby, Adel S. 

作者机构：Univ Louisville Dept Psychiat Louisville KY 40202 USA Univ Louisville Dept Comp Sci & Comp Engn Louisville KY 40292 USA 

出 版 物：《JOURNAL OF NEUROSCIENCE METHODS》 (神经科学方法杂志)

年 卷 期：2008年第168卷第2期

页      面：367-372页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：NIMH NIH HHS [MH62654  R01 MH069991  R01 MH062654-04  R01 MH062654  MH69991  R01 MH069991-04] Funding Source: Medline 

主　　题：computerized image analysis minicolumns myelin neocortex pyramidal cells 

摘      要：Minicolumns are thought to be the smallest cortical modules within the hierarchical organization of the isocortex. Several reports suggest alterations in minicolumnar morphometry may be involved in psychiatric disorders such as autism, dyslexia, and schizophrenia. Thus far anatomical studies of minicolumns have primarily relied on measurements of pyramidal cell arrays. This study expands on a recursive trace line segmentation method used to define morphometric measures for myelinated axon bundles. The results were compared against those of pyramidal cell arrays derived from immediately adjacent serial sections. Width estimates based on cell somas and myelinated axon bundles were highly correlated (r = 0.9888). Histograms of signal intensity using the recursive trace line method produced expected features of myeloarchitectonics;that is, bundles of Meynert and intervening interradiary plexus. The close correspondence of derived values for myelinated axon bundles and pyramidal cell arrays suggests their participation and interaction within the same modular arrangement of the isocortex. (c) 2007 Elsevier B.V. All rights reserved.