Resveratrol prevents hepatic steatosis and endoplasmic reticulum stress and regulates the expression of genes involved in lipid metabolism, insulin resistance, and inflammation in rats

作     者：Pan, Qing-Rong Ren, Yan-Long Liu, Wen-Xian Hu, Yan-Jin Zheng, Jin-Su Xu, Yuan Wang, Guang 

作者机构：Capital Med Univ Beijing Chaoyang Hosp Dept Endocrinol Beijing 100020 Peoples R China Capital Med Univ Beijing Anzhen Hosp Dept Cardiol Beijing Inst Heart Lung & Blood Vessel Dis Beijing 100029 Peoples R China Capital Med Univ Beijing Chaoyang Hosp Dept Tradit Chinese Med Beijing 100020 Peoples R China 

出 版 物：《NUTRITION RESEARCH》 (营养研究)

年 卷 期：2015年第35卷第7期

页      面：576-584页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 120402[管理学-社会医学与卫生事业管理(可授管理学、医学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：National Natural Science Foundation of China [81100587, 81270369, 81070244] Beijing Natural Science Foundation [1142001, 7112044] 

主　　题：Resveratrol Hepatic steatosis Endoplasmic reticulum stress PCR array Rat 

摘      要：Previous research demonstrated that resveratrol possesses promising properties for preventing obesity. Endoplasmic reticulum (ER) stress was proposed to be involved in the pathophysiology of both obesity and hepatic steatosis. In the current study, we hypothesized that resveratrol could protect against high-fat diet (HFD)-induced hepatic steatosis and ER stress and regulate the expression of genes related to hepatic steatosis. Rats were fed either a control diet or a HFD for 12 weeks. After 4 weeks, HFD-fed rats were treated with either resveratrol or vehicle for 8 weeks. Body weight, serum metabolic parameters, hepatic histopathology, and hepatic ER stress markers were evaluated. Moreover, an RT2 Profiler Fatty Liver PCR Array was performed to investigate the mRNA expressions of 84 genes related to hepatic steatosis. Our work showed that resveratrol prevented dyslipidemia and hepatic steatosis induced by HFD. Resveratrol significantly decreased activating transcription factor 4, C/EBP-homologous protein and immunoglobulin binding protein levels, which were elevated by the HFD. Resveratrol also decreased PKR-like ER kinase phosphorylation, although it was not affected by the HFD. Furthermore, resveratrol increased the expression of peroxisome proliferator-activated receptor delta, while decreasing the expression of ATP citrate lyase, suppressor of cytokine signaling-3, and interleukin-1 beta. Our data suggest that resveratrol can prevent hepatic ER stress and regulate the expression of peroxisome proliferator-activated receptor delta, ATP citrate lyase, suppressor of cytokine signaling-3, tumor necrosis factor alpha, and interleukin-1 beta in diet-induced obese rats, and these effects likely contribute to resveratrol s protective function against excessive accumulation of fat in the liver. (C) 2015 Published by Elsevier Inc.