The associations of membrane systems

作     者：Bourd, G.I. Martirosov, S.M. 

作者机构：The Gamaleya Institute for Epidemiology and Microbiology Academy of Medical Sciences Russian Federation Armenian Branch of VNII Genetika Box 45 Genetika Box 45 Genetika Russian Federation 

出 版 物：《Journal of Electroanalytical Chemistry》 (J Electroanal Chem)

年 卷 期：1983年第155卷第C期

页      面：315-333页

主　　题：DCCD N,N′-dicyclohexylcarbodiimide Δμ ̄ is the electrochemical potential difference δψ is the membrane potential F 1 F 0 is the ATPase complex for H + transport F 1 is the ATPase F 0 the proteolipid incorporated in a membrane TrkA is the system for transfer of K + TrkF is the H + /K + -exchanging system ΔpH is H + gradients Δp K is K + gradients PTS is the phosphoenolpyruvate-dependent carbohydrate phosphotransferase system PEP is phosphoenolpyruvate MG is methyl-α- d -glucopyranoside AC is adenylate cyclase M-protein is β-galactoside permease product of gene lac Y ONPG is o -nitrophenyl-β- d -galactopyranoside NEM is N-ethylmaleimide TDG is thiodigalactoside E II is the membrane component of the PTS 

摘      要：This review is devoted to the data on the intramembranal interactions of proteins for the regulation of membrane transport, of cell metabolism anf of energy transformation. First discussed are the results on H+ transfer via the ATPase complex F1F0 and the counter-transport of K+ in glycolysing bacteria. It is concluded that the F1F0 and the natural K+ ionophore are able to form an intramembranal binary supercomplex for the joint employment of ATP energy. Such a supercomplex operates like an ionic pump, exchanging 2 H+ for K+ and capable of both hydrolysis and synthesis of ATP with the essential decrease of energy dissipation. It is also assumed that such a system in gram-negative bacteria includes, as a third portion, a protein regulator which turns the H+/K+ exchange on and off dependent on osmotic pressure in the medium. The data on H+/K+ antiport in bacteria are also considered. In this case the possibility of the formation of another type of supercomplex formed by the proton channel F0 and the K+ ionophore is suggested. Thus, the interaction of membrane proteins for the joint employment of converted energy ATP, Δμ ̄H and Δμ ̄K is considered. In the second part of the review the results on the cell metabolism regulation through the intramembranal interaction of some systems in bacteria are described. These regulations are performed via the mechanisms known as inducer exclusion and catabolite repression. The reciprocal inhibiting effect under the interplay of PTS and lactose permease is shown. The lactose permease is assumed to be inoperative due to the interaction with E11 when the latter translocates glucose (or mannose). Some experiments serve as the evidence that the catabolite repression connected with the fall in adenylate cyclase activity occurs at membrane l