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作者机构:第三军医大学西南医院神经内科重庆400038 第三军医大学西南医院神经外科全军神经系统疾病微创诊治专科中心重庆400038 第三军医大学西南医院麻醉科重庆400038
出 版 物:《第三军医大学学报》 (Journal of Third Military Medical University)
年 卷 期:2009年第31卷第7期
页 面:581-584页
核心收录:
学科分类:1008[医学-中药学(可授医学、理学学位)] 100703[医学-生药学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金:国家自然科学基金(30500662 30800354)
主 题:蜕皮甾酮 局灶性脑缺血 血管内皮生长因子 血管生成 神经功能
摘 要:Objective To observe the effect of ecdysterone(EDS)on the level of VEGF protein in the brain,angiogenesis and neurologic function after focal cerebral ischemia in *** Rat with focal cerebral ischemia were established by occluding their middle cerebral *** established rats(n=36)were randomly and equally divided into EDS treatment group and ischemia ***(20 mg·kg-1·d-1 for 7 d)was intraperitoneally injected into the rats of EDS treatment group 2 h after operation,and the animal of ischemia group received an intraperitoneal injection of the same solvent as in EDS *** 6 rats served as normal *** were sacrificed in 7,14 and 21 d after operation,and the VEGF protein level and microvessel density(MVD)was detected with immunohistochemical methods and analyzed quantitatively with image *** of EDS on neurologic recovery following brain ischemia were assessed using the neurologic severity scores(NSS).Results VEGF expression was not seen in normal control,and was higher in ischemia group than in the EDS treatment group at day 7 and 14,but the significant difference was only observed at day 7(P0.01).VEGF expression was disappeared at day 21 in ischemia *** treatment group had signi-ficantly higher MVD than in the ischemia group at all time *** results indicated middle cerebral artery occlusion led significant neurologic impairment and subsequently certain degrees of autogenous recovery were *** of these indices was significantly better in the EDS treatment group than in ischemia group at day 7(P0.05)and 14(P0.01).Conclusion EDS can definitely induce VEGF expression and angiogenesis in areas around ischemic brain regions in rats with focal cerebral ischemia,and promote neurologic recovery following the injury.