Multiresponse optimization of inoculum conditions for the production of amylases and proteases by aspergillus awamori in solid-state fermentation of babassu cake

作     者：Castro, Aline Machado de Teixeira, Mariana Martins Pereira Carvalho, Daniele Fernandes Freire, Denise Maria Guimarães Castilho, Leda Dos Reis 

作者机构：Biotechnology Division Research and Development Center PETROBRAS Ilha do Fundão 21941-915 Rio de Janeiro RJ Avenide Horácio Macedo 950 Brazil COPPE Chemical Engineering Program Federal University of Rio de Janeiro 21941-972 Rio de Janeiro RJ Brazil Institute of Chemistry Federal University of Rio de Janeiro 21945-970 Rio de Janeiro RJ Brazil 

出 版 物：《Enzyme Research》 (Enzyme Res.)

年 卷 期：2011年第2011卷第1期

页      面：457392页

学科分类：0710[理学-生物学] 08[工学] 09[农学] 0703[理学-化学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

摘      要：This work aimed at investigating the simultaneous production of amylases and proteases by solid-state fermentation (SSF) of babassu cake using Aspergillus awamori IOC-3914. By means of experimental design techniques and the desirability function, optimum inoculum conditions (C/N ratio of propagation medium, inoculum age, and concentration of inoculum added to SSF medium) for the production of both groups of enzymes were found to be 25.8, 28.4 h, and 9.1 mg g-1, respectively. Significant influence of both initial C/N ratio and inoculum concentration was observed. Optimum amylolytic activities predicted by this multiresponse analysis were validated by independent experiments, thus indicating the efficacy of this approach. © 2011 Aline Machado de Castro et al.