Assessing the Thrombogenic Potential of Heart Valve Prostheses: An Approach for a Standardized In-Vitro Method

作     者：Linde, Torsten Clauser, Johanna Meuris, Bart Steinseifer, Ulrich 

作者机构：Rhein Westfal TH Aachen Helmholtz Inst Appl Med Engn Dept Cardiovasc Engn Aachen Germany Monash Univ Monash Inst Med Engn Dept Mech & Aerosp Engn Fac Engn Melbourne Vic Australia Katholieke Univ Leuven Univ Hosp Leuven Dept Cardiovasc Sci Cardiac Surg Leuven Belgium 

出 版 物：《CARDIOVASCULAR ENGINEERING AND TECHNOLOGY》 (心血管工程技术)

年 卷 期：2019年第10卷第2期

页      面：216-224页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：German Research Foundation (DFG) [SCHM1307/5-1] 

主　　题：Heart valve prostheses Thrombosis In vitro testing Tester validation 

摘      要：PurposeThrombogenic complications are still a main issue in the general performance of cardiovascular implants, especially heart valves. To date, the thrombogenic potential of those prostheses is pre-clinically assessed in time consuming animal studies with questionable *** this study, we present a new in-vitro method to assess and compare deficiencies of heart valve substitutes concerning their thrombogenic performance and locate initial clot formation under physiological conditions using porcine blood. Therefore, an athrombogenic pulse duplicator (THIA3) was developed that simulates the anatomic and hemodynamic conditions in the vicinity of the aortic valve. Validation of this tester was carried out with regard to hemodynamics, reproducibility and using positive and negative control valves and by comparison of clot locations with literature data from chronic animal trials with sheep using a St. Jude bileaflet *** of the tester showed quasi-physiological hemodynamics and reproducible clot formation. Identical clot formations were found comparing findings in vitro with chronic animal *** THIA 3 has proven its suitability for valid, reproducible evaluation of thrombogenic potential of heart valves in a short period of time.