Pilot scale production of pory(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli

作     者：Choi, Jong-Il Lee, Sang Yup Shin, Kyungsup Lee, Woo Gi Park, Si Jae Chang, Ho Nam Chang, Yong Keun 

作者机构：Metabolic and Biomolecular Engineering National Research Laboratory Korea Advanced Institute of Science and Technology Yuseong-gu Daejeon 305-701 373-1 Guseong-dong South Korea Department of Chemical and Biomolecular Engineering BioProcess Engineering Research Center Yuseong-gu Daejeon 305-701 373-1 Guseong-dong South Korea Center for Ultramicrochemical Process Systems Korea Advanced Institute of Science and Technology Yuseong-gu Daejeon 305-701 373-1 Guseong-dong South Korea 

出 版 物：《Biotechnology and Bioprocess Engineering》 (Biotechnol. Bioprocess Eng.)

年 卷 期：2002年第7卷第6期

页      面：371-374页

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 0836[工学-生物工程] 0702[理学-物理学] 

主　　题：Oxygen transfer rate P(3HB/V) Pilot-scale fermentor Recombinant E. coli 

摘      要：Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having a KLa value of 0.11 s-1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a KLa of 0.03 s-1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.