基于银增强金标记物的阳极溶出伏安免疫分析用于补体第三成分的测定

作     者：楚霞 傅昕 沈国励 俞汝勤 CHU Xia;FU Xin;SHEN Guo-Li;YU Ru-Qin

作者机构：湖南师范大学化学化工学院长沙410081 湖南大学化学与生物传感及化学计量学国家重点实验室长沙410082 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：2005年第26卷第9期

页      面：1637-1639页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：国家自然科学基金(批准号:20105007)资助 

主　　题：金属免疫分析 阳极溶出伏安法 银增强溶液 免疫金标记物 

摘      要：A novel, sensitive anodic stripping volammetric immunoassay was developed based on silver-enhanced immuno-gold label. The immunoreaction was performed in a polystyrene microwell by using the sandwich format. Primary antibodies specific for C-3(complement Ⅲ) were adsorbed passively on the walls of a polystyrene microwell. The C-3 analyte was first captured by the primary antibody and then sandwiched by a secondary colloidal gold-labeled antibody. The addition of the silver enhancement solution results in the precipitation of a large amount of silver on colloidal gold labels due to the catalytic reduction which, after silver metal dissolution in an acidic solution, was determined by anodic stripping voltammetry(ASV) at a glassy-carbon electrode. The influence of some immunoassay conditions upon the anodic stripping peak current was examined and optimized. The anodic stripping peak current depended linearly on the logarithm of C-3 mass concentration over the range of 7.2 ng/mL to 7.33 μg/mL and a detection limit as low as 7ng/mL is achieved. The anodic stripping volammetric immunoassay was applied to the determination of C-3 concentration in human serum with satisfactory results.