Sex-linked microsatellite marker detected in the female gametophytes of <i>Undaria pinnatifida</i> (Phaeophyta)

作     者：Shan, Ti Feng Pang, Shao Jun 

作者机构：Chinese Acad Sci Inst Oceanol Marine Biol Culture Collect Ctr Qingdao 266071 Peoples R China 

出 版 物：《PHYCOLOGICAL RESEARCH》 (藻类学研究)

年 卷 期：2010年第58卷第3期

页      面：171-176页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：863 Hi-Tech Research and Development Program of China [2006AA10A412] Natural Science Foundation of China Chinese Academy of Sciences [KSCX2-YW-N-47-07] IOCAS Ministry of Agriculture of China 

主　　题：gametophyte microsatellite marker sex determination sex-related Undaria pinnatifida 

摘      要：PIn our microsatellite analysis of three male and three female gametophytes of Undaria pinnatifida (Harv.) Suringar, a microsatellite marker (part of the locus Up-AC-2A8, GenBank accession no. AY738602.1) was only polymerase chain reaction-amplified in three female gametophytes. This putative female-specific marker was further tested by the use of 32 male and 21 female gametophytes maintained in the Marine Biological Culture Collection Centre, China. In addition, three sporophytes were included for confirmation. Results showed that the marker was present in all of the female gametophytes and sporophyte cultures, but absent in all of the male gametophytes. To our knowledge, this is the first sex-related marker ever reported in U. pinnatifida. The discovery of this marker will accelerate gender identification and shed light on our understanding of the mechanisms of sex determination at a molecular level in this commercially important seaweed.