MITOCHONDRIAL PROTEIN IMPORT - DIFFERENTIAL RECOGNITION OF VARIOUS TRANSPORT INTERMEDIATES BY ANTIBODIES

作     者：SOLLNER, T PFANNER, N NEUPERT, W 

作者机构：Institut für Physiologische Chemie Universität München FRG. 

出 版 物：《FEBS LETTERS》 (欧洲生化学会联合会快报)

年 卷 期：1988年第229卷第1期

页      面：25-29页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：Mitochondria Protein import ADP/ATP carrier F 1 -ATPase subunit β 

摘      要：The precursors of the mitochondrial proteins ADP/ATP carrier (AAC) and F1-ATPase subunit beta (F1 beta) were accumulated at the stages of binding to receptor sites on the mitochondrial outer membrane, or in contact sites between outer and inner membranes. Specific antibodies raised against the mature proteins were added to the isolated mitochondria and efficiently bound to these translocation intermediates. Further movement of the precursors to consecutive steps along their import pathway was thereby inhibited. Controls showed that precursor proteins which were inserted into or translocated across the outer membrane were not recognized by the antibodies unless the mitochondrial membranes were disrupted. We conclude that the trapped translocation intermediates have antigenic sites exposed to the outside of the outer membrane.