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作者机构:南京农业大学农业部动物疫病诊断与免疫重点开放实验室江苏南京210095
出 版 物:《中国病毒学》 (Virologica Sinica)
年 卷 期:2005年第20卷第4期
页 面:441-443页
核心收录:
学科分类:090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
基 金:国家"863"高技术发展计划资助项目(2001AA249012)
主 题:猪繁殖与呼吸综合症病毒 GP5蛋白 原核表达
摘 要:The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a(+) , the recombinant plasmid named pET-GP5 was transformed into *** cell BL21. SDS-PAGE and Western-blot indicated that the orf5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV.