Characterization of Cytosolic V_1-ATPase in Pea Root

作     者：余和芬 陈珈 王学臣 YU He-Fen;CHEN Jia;WANG Xue-Chen

作者机构：中国农业大学生物学院植物生理生化国家重点实验室 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2003年第45卷第2期

页      面：164-168页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：国家重点基础研究发展规划项目 (G19990 1170 0 ) 国家自然科学基金 (3 9670 0 76) 生物大分子国家重点实验室基金(881611)资助 

主　　题：V-type H+ -ATPase (V-ATPase) V-1-ATPase pea 

摘      要：Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.