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出 版 物:《Acta Botanica Sinica》 (Acta Botanica Sinica(植物学报:英文版))
年 卷 期:2003年第45卷第2期
页 面:164-168页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 071009[理学-细胞生物学]
基 金:国家重点基础研究发展规划项目 (G19990 1170 0 ) 国家自然科学基金 (3 9670 0 76) 生物大分子国家重点实验室基金(881611)资助
主 题:V-type H+ -ATPase (V-ATPase) V-1-ATPase pea
摘 要:Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.