Growth cone and dendrite dynamics in zebrafish embryos:: early events in synaptogenesis imaged <i>in vivo</i>

作     者：Jontes, JD Buchanan, J Smith, SJ 

作者机构：Stanford Univ Sch Med Dept Cellular & Mol Physiol Stanford CA 94305 USA 

出 版 物：《NATURE NEUROSCIENCE》 (自然神经科学)

年 卷 期：2000年第3卷第3期

页      面：231-237页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071003[理学-生理学] 

基　　金：National Institutes of Health  NIH 

主　　题：轴突/代谢 轴突/超微结构 细胞运动 细胞大小 树突/代谢 胚胎 非哺乳动物/细胞学 荧光染料 生长锥/代谢 显微镜检查 电子 显微镜检查 荧光 运动神经元/细胞学 运动神经元/代谢 运动神经元/超微结构 伪足/代谢 脊髓/细胞学 脊髓/胚胎学 突触/代谢 突触/超微结构 突触小泡/超微结构 时间因素 斑马鱼/胚胎学 动物 

摘      要：We used time-lapse fluorescence microscopy to observe the growth of Mauthner cell axone and their postsynaptic targets, the primary motor neurons, in spinal cords of developing zebrafish embryos. Upon reaching successive motor neurons, the Mauthner growth cone paused briefly before continuing along its path. Varicosities formed at regular intervals and were preferentially associated with the target regions of the primary motor neurons. In addition, the postsynaptic motor neurons showed highly dynamic filopodia, which transiently interacted with both the growth cone and the axon. Both Mauthner cell and motor neurons were highly active, each showing motility sufficient to initiate synaptogenesis.