Anion exchange polymeric sorbent coupled to high-performance liquid chromatography with UV diode array detection for the determination of ten N-nitrosamines in meat products: a validated approach

作     者：Iammarino, Marco Mangiacotti, Michele Chiaravalle, Antonio E. 

作者机构：Ist Zooprofilatt Sperimentale Puglia & Basilicata Via Manfredonia 20 I-71121 Foggia Italy 

出 版 物：《INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY》 (国际食品科学与技术杂志)

年 卷 期：2020年第55卷第3期

页      面：1097-1109页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 

基　　金：Italian Ministry of Health [GR-2013-02358862] 

主　　题：Anion exchange sorbent carcinogenic compounds diode array detection food safety high-performance liquid chromatography meat nitrosamines processed meats solid-phase extraction validation 

摘      要：Among mechanisms and molecules presumably involved in the carcinogenicity induced by meat consumption, the N-nitrosamines (N-NAs) are a class of compounds characteristic of processed meats. In this work, an analytical method for the determination of ten N-nitrosamines (N-nitrosomorpholine, N-nitrosomethylethylamine, N-nitrosopyrrolidine, N-nitrosodiethylamine, N-nitrosopiperidine, N-nitrosodipropylamine, N-nitrosodimethylaniline, N-nitrosodibutylamine, N-nitrosodiphenylamine and N-nitrosodibenzylamine) in fresh meats and meat products was developed, optimised and validated. The method is based on optimised sample purification by solid-phase extraction (anion exchange polymeric sorbent) and separation/detection by high-performance liquid chromatography coupled to UV diode array detection. The validation procedure allowed to ascertaining good analytical performances in terms of sensitivity, selectivity towards interfering compounds, accuracy and robustness. The values obtained for precision (range: 4.3-14.4%) and recovery percentages (range: 80.8-95.1%) were compared to reference values indicated in the Decision No. 657/2002/EC, resulting as compliant. The measurement uncertainty (lower than 14.6%) was satisfactory for each N-NA as well.