Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3

作     者：REN Shiying LI Xiangqian YIN Xiulian LUO Chuping LIU Fei REN Shiying;LI Xiangqian;YIN Xiulian;LUO Chuping;LIU Fei

作者机构：Jiangsu Provincial Key Construction Laboratory of Probiotics PreparationHuaiyin Institute of TechnologyHuai’an 223003China Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical IndustryHuaiyin Institute of TechnologyHuai’an 223003China School of Life Science and Food EngineeringHuaiyin Institute of TechnologyHuai’an 223003China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2020年第38卷第1期

页      面：195-203页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：Supported by the National Natural Science Foundation of China(Nos.51308245,31570061) the Open Project of Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical Industry(No.SF201408) the Open Project of Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation(No.JSYSZJ2017006) 

主　　题：polyphosphate-accumulating bacterium polyphosphate granule Halomonas enhanced biological phosphorus removal 

摘      要：Halomonas ***-3 was isolated from the Yellow Sea and identified as a polyphosphate-accumulating bacterium and the characteristics of its intracellular polyphosphate(polyP)granules and phosphorus absorption were *** YSR-3 cells stored one or two polyP granules in regular appearance and *** diameter of the granules was about 400 nm measuring by a transmission electron microscope(TEM).After stained with 4,6-diamidino-2-phenylindole(DAPI)and visualized by a fluorescence microscope,the cells turned blue and the granules were bright *** composition of granules includes P(major ingredient),Mg,S,K,and Ca as detected by an energy dispersive X-ray spectrometer(EDS).When inorganic phosphorus(po34-)and ferric ion(Fe3+)were added into media,the biomass increased and the cells formed intracellular polyP granules owing to the phosphorus assimilation from *** YSR-3 obtained higher biomass by adding 0.02 g/L FePO4 than 0.005 g/L and 0.01 g/L FePO4;however,the phosphorus absorption was higher with 0.01 g/L FePO4 than 0.005 g/L and 0.02 g/L *** optical density at wavelength 480 nm(OD480nm)was 0.79 and 100%cells could form intracellular polyP *** results show that strain YSR-3 is able to acquire higher biomass and absorb more inorganic phosphorus when 0.01 g/L FePO4 is *** characteristics of absorbing and storing phosphorus as intracellular inorganic polyP granules have a potential for application in high-efficiency phosphorus removal in wastewater treatment.