Thioredoxin-Interacting Protein(TXNIP) Regulates Parkin/PINK1-mediated Mitophagy in Dopaminergic Neurons Under High-glucose Conditions: Implications for Molecular Links Between Parkinson’s Disease and Diabetes

作     者：Cun-Jin Su Zhu Shen Ru-Xiao Cui Ya Huang De-Lai Xu Feng-Lun Zhao Jie Pan Ai-Ming Shi Tong Liu Yun-Li Yu Cun-Jin Su;Zhu Shen;Ru-Xiao Cui;Ya Huang;De-Lai Xu;Feng-Lun Zhao;Jie Pan;Ai-Ming Shi;Tong Liu;Yun-Li Yu

作者机构：Department of PharmacyThe Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Institute of NeuroscienceSoochow UniversitySuzhou 215004China Department of Clinical PharmacologyThe Second Affiliated Hospital of Soochow UniversitySuzhou 215004China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2020年第36卷第4期

页      面：346-358页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100204[医学-神经病学] 10[医学] 

基　　金：the National Science Foundation of China(81601098 and 81603181) the Natural Science Foundation of Jiangsu Province(BK20150302,BK20170004) the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJB310016) Suzhou Science and Technology for People's Livelihood(SYS201706) the Natural Science Foundation of Suzhou(SYSD2018099) 

主　　题：Diabetes mellitus Parkinson’s disease High glucose TXNIP Mitophagy PC12 cells 

摘      要：Patients with diabetes mellitus have a higher risk of developing Parkinson’s disease(PD). However, the molecular links between PD and diabetes remain *** this study, we investigated the roles of thioredoxininteracting protein(TXNIP) in Parkin/PINK1-mediated mitophagy in dopaminergic(DA) cells under high-glucose(HG) conditions. In streptozotocin-induced diabetic mice,TXNIP was upregulated and autophagy was inhibited in the midbrain, while the loss of DA neurons was accelerated by hyperglycemia. In cultured PC12 cells under HG, TXNIP expression was upregulated and the intracellular reactive oxygen species(ROS) levels increased, leading to cell death. Autophagic flux was further blocked and PINK1 expression was decreased under HG conditions. Parkin expression in the mitochondrial fraction and carbonyl cyanide 3-chlorophenylhydrazone(CCCP)-induced co-localization of COX IV(marker for mitochondria) and LAMP1(marker for lysosomes) were also significantly decreased by HG. Overexpression of TXNIP was sufficient to decrease the expression of both PINK1 and Parkin in PC12 cells, while knockdown of the expression of TXNIP by si RNA decreased intracellular ROS and attenuated cellular injury under HG. Moreover, inhibition of TXNIP improved the CCCP-induced co-localization of COX IV and LAMP1 in PC12 cells under HG. Together, these results suggest that TXNIP regulates Parkin/PINK1-mediated mitophagy under HG conditions, and targeting TXNIP may be a promising therapeutic strategy for reducing the risk of PD under hyperglycemic conditions.