ENERGETICS OF PHOTOSYNTHESIS IN ZEA-MAYS .2. STUDIES OF THE FLASH-INDUCED ELECTROCHROMIC SHIFT AND FLUORESCENCE INDUCTION IN MESOPHYLL CHLOROPLASTS

作     者：CROWTHER, D LEEGOOD, RC WALKER, DA HIND, G 

作者机构：UNIV SHEFFIELD DEPT BOT ARC PHOTOSYTHN RES GRP SHEFFIELD S10 2TN S YORKSHIRE ENGLAND BROOKHAVEN NATL LAB DEPT BIOL UPTON NY 11973 USA 

出 版 物：《BIOCHIMICA ET BIOPHYSICA ACTA》 (Biochim. Biophys. Acta Bioenerg.)

年 卷 期：1983年第722卷第1期

页      面：127-136页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：Photosynthesis Electron transport Chlorophyll fluorescence Fluorescence induction (Zea mays mesophyll chloroplast) DCMU 3-(3,4-dichlorophenyl)-1, 1-dimethylurea Mes 2-( N -morpholino)ethanesulphonic acid Tricine N -tris(hydroxymethyl)methylglycine F 0 intrinsic bed fluorescence F i initial level of fluorescence seen during rapid induction ( F 0 plus a variable component proportional to the state of reduction of Q) F m maximal level of fluorescence (seen in the presence of DCMU) F v variable fluorescence (maximally F m − F 0 ) Chl chlorophyll PS photosystem 

摘      要：Patterns of electron transfer in isolated mesophyll chloroplasts of maize (Z. mays L.) were studied in the presence of the physiological substrates, oxaloacetate, 3-phosphoglycerate and pyruvate. Flash-induced absorbance changes due to the electrochromic pigment band-shift (P-518) were used to estimate relative electron flow rates through the cyclic and noncyclic pathways of electron transport. Further information on the redox state of electron carriers and the activity of coupled electron flow was obtained from measurements of fluorescence induction and of actinic-light-induced fluorescence changes. The importance of correct redox poising for optimal rates of photosynthesis was demonstrated and the operation and regulation of photosynthesis in the C4 system discussed.