Measuring maximum oxygen uptake with an incremental swimming test and by chasing rainbow trout to exhaustion inside a respirometry chamber yields the same results

作     者：Zhang, Yangfan Gilbert, Matthew J. H. Farrell, Anthony P. 

作者机构：Univ British Columbia Dept Zool Vancouver BC V6T 1Z4 Canada Univ British Columbia Fac Land & Food Syst Vancouver BC V6T 1Z4 Canada 

出 版 物：《JOURNAL OF FISH BIOLOGY》 (鱼类生物学杂志)

年 卷 期：2020年第97卷第1期

页      面：28-38页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 09[农学] 

基　　金：Natural Sciences and Engineering Research Council of Canada, NSERC Canada Research Chairs University of British Columbia, UBC 

主　　题：chasing critical swimming speed dynamic & x1e40 O-2 exhaustion iterative algorithm minimum sampling window MMR rainbow trout respirometry 

摘      要：This study hypothesized that oxygen uptake (O-2) measured with a novel protocol of chasing rainbow troutOncorhynchus mykissto exhaustion inside a static respirometer while simultaneously monitoring O-2(O-2chase) would generate the same and repeatable peak value as when peak active O-2(O-2active) is measured in a critical swimming speed protocol. To reliably determine peak O-2chase, and compare to the peak during recovery of O(2)after a conventional chase protocol outside the respirometer (O-2rec), this study applied an iterative algorithm and a minimum sampling window duration (i.e., 1 min based on an analysis of the variance in background and exercise O-2) to account for O(2)dynamics. In support of this hypothesis, peak O-2active(707 +/- 33 mg O(2)h(-1)kg(-1)) and peak O-2chase(663 +/- 43 mg O(2)h(-1)kg(-1)) were similar (P= 0.49) and repeatable (Pearson s and Spearman s correlation test;r = 0.77;P 0.05) when measured in the same fish. Therefore, estimates of O(2max)can be independent of whether a fish is exhaustively chased inside a respirometer or swum to fatigue in a swim tunnel, provided O(2)is analysed with an iterative algorithm and a minimum but reliable sampling window. The importance of using this analytical approach was illustrated by peak O(2chase)being 23% higher (P 0.05) when compared with a conventional sequential interval regression analysis, whereas using the conventional chase protocol (1-min window) outside the respirometer increased this difference to 31% (P 0.01). Moreover, because peak O(2chase)was 18% higher (P 0.05) than peak O-2rec, chasing a fish inside a static respirometer may be a better protocol for obtaining maximum O-2.