Investigation of bone matrix composition, architecture and mechanical properties reflect structure-function relationship of cortical bone in glucocorticoid induced osteoporosis

作     者：Xi, Li Song, Yu Wu, Wenwang Qu, Zhaoliang Wen, Jiawei Liao, Binbin Tao, Ran Ge, Jingran Fang, Daining 

作者机构：Beijing Inst Technol Inst Adv Struct Technol Beijing Peoples R China Queen Mary Univ London Sch Engn & Mat Sci London E1 4NS England Diamond Light Source Ltd Beamline I22Harwell Sci & Innovat Campus Didcot Oxon England North Carolina State Univ Coll Text Raleigh NC USA Shanghai Jiao Tong Univ Sch Naval Architecture Ocean & Civil Engn Dept Engn Mech Shanghai 200240 Peoples R China MIT Dept Mech Engn Cambridge MA 02139 USA Univ Moratuwa Dept Mech Engn Moratuwa Sri Lanka Peking Univ Coll Engn State Key Lab Turbulence & Complex Syst Beijing Peoples R China 

出 版 物：《BONE》 (骨)

年 卷 期：2020年第136卷

页      面：115334-115334页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Diamond Light Source [SEML1B4R] Queen Mary University of London Medical Research Council [G0600702] China Scholarship Council China Postdoctoral Science Foundation [2019M660476] National Natural Science Foundation of China [11702023, 11802018] Beijing Natural Science Foundation Diamond Light Source (Harwell, UK) 

主　　题：Glucocorticoid induced osteoporosis Structure-function relationships Nanoindentation Synchrotron X-ray nanomechanical imaging 

摘      要：Glucocorticoid induced osteoporosis (GIOP) is the most common negative consequence of long-term glucocorticoid treatment, leading to increased fracture risk followed by loss of mobility and high mortality risk. These biologically induced changes in bone quality at molecular level lead to changes both in bone matrix architecture and bone matrix composition. However, the quantitative details of changes in bone quality - and especially their link to reduced macroscale mechanical properties are still largely missing. In this study, a mouse model for glucocorticoid-induced osteoporosis (GIOP) was used to investigate mechanical and material alterations in bone cortex (natural nanocomposite) at different scale. By combining quantitative backscattered electron (qBSE) imaging, nanoindentation and high brilliance synchrotron X-ray nanomechanical imaging on a genetically modified mouse model of GIOP, we were able to quantify the local indentation modulus, mineralization distribution and the alterations of nanoscale structures and deformation mechanisms in the mid-diaphysis of femur, and relate them to the macroscopic mechanical changes. Our results showed clear and significant changes in terms of material quality of bone at nanoscale and microscale, which manifests itself in development of spatial heterogeneities in mineralization and indentation moduli across the bone organ, with potential implications for increased fracture risk.