Transcriptomic analysis reveals the regulation network of BmKruppel homolog 1 in the oocyte development of Bombyx mori

作     者：Zi-Dan Zhu Qi-Hao Hu Chun-Mei Tong Hong-Guang Yang Si-Chun Zheng Qi-Li Feng Hui-Min Deng Zi-Dan Zhu;Qi-Hao Hu;Chun-Mei Tong;Hong-Guang Yang;Si-Chun Zheng;Qi-Li Feng;Hui-Min Deng

作者机构：Guangdong Key Laboratory of Insect Developmental Biology and Applied TechnologyGuangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2021年第28卷第1期

页      面：47-62页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：the grants from the Chinese National Natural Science Foundation(No.31872969,31672494 and 31330071) Guangdong Provincial Natural Science Foundation(No.2017A030306003) Guangdong Provincial Special Support Program(No.2017TQ04N744) Pearl River S and T Nova Program of Guangzhou(201710010037) 

主　　题：Kriippel homolog 1 oocyte development regulatory network RNA interference transcriptomics 

摘      要：Kriippel homolog 1(Kr-hl),a zinc finger transcription factor,is involved in the metamorphosis and adult reproduction of ***,the role of Kr-hl in reproduction of holometabolic insects remains to be *** regulation network of 7-associated genes in the reproduction in Bombyx mori was investigated in this *** higher expression level of BmKr-hl in the ovaries was detected during the late pupal stage and *** interference(RNAi)-mediated depletion of BmKr-hl in the female at day 6 of pupae resulted in abnormal oocytes at 48 h post-double-stranded RNA treatment,which showed less yolk protein deposition and partially transparent ***-seq and subsequent differentially expressed transcripts analysis showed that knockdown of BmKr-hl caused a decrease in the expression of 2882 genes and an increase in the expression of 2565 genes in the oocytes at day 8 of ***,27 genes coding for transcription factors were down-regulated,while six genes coding for other transcription factors were ***-hl bound to the Kr-hl binding site of the transcription factors AP-1(activating protein-1)and FOXG1 to increase their messenger RNA tran・scripts in the BmN cells,*** Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of that positively co-expressed with AP-1 and FOXG1 transcripts showed mainly enrichment in the metabolic-related pathways,the nu・tri ent absorption and the yolk protein absorption *** data suggested that BmKr-h 1 might directly regulate the metabolic-related pathways,the nutrient absorption and the yolk protein absorption processes or probably through AP-1 and/or FOXG1 to regulate oocyte development.