Induction of diabetes in cynomolgus monkey with one shot of analytical grade streptozotocin

作     者：Zhengzhao Liu Ying Lu Wenbao Hu Hidetaka Hara Yifan Dai Zhiming Cai Lisha Mou Zhengzhao Liu;Ying Lu;Wenbao Hu;Hidetaka Hara;Yifan Dai;Zhiming Cai;Lisha Mou

作者机构：Shenzhen Xenotransplantation Medical Engineering Research and Development CenterInstitute of Translational MedicineShenzhen Second People's HospitalThe First Affiliated Hospital of Shenzhen UniversityHealth Science CenterShenzhenChina Movement System Injury and Repair Research CenterXiangya Hospital of Central South UniversityChangshaChina Jiangsu Key Laboratory of XenotransplantationNanjing Medical UniversityNanjingChina Xenotransplantation Program/Department of SurgeryThe University of Alabama at BirminghamBirminghamALUSA 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2020年第3卷第1期

页      面：79-86页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This work were supported by grants from the National Key R&D Program of China(2017YFC1103704) Special Funds for the Construction of High Level Hospitals in Guangdong Province(2019),Sanming Project of Medicine in Shenzhen(SZSM201412020) Fund for High Level Medical Discipline Construction of Shenzhen(2016031638) National Science Foundation for Distinguished Yong Scholars of Guangdong province(2016A030306051) National Science Foundation(81701383) National Science Foundation for Postdoctoral Startup of Guangdong province(2017A030310005) China Postdoctoral Science Foundation(2015M580755) China Postdoctoral Science Special Foundation(2016T90813) Shenzhen Foundation of Health and Family Planning Commission(SZXJ2017021) 

主　　题：analytical grade diabetic model nonhuman primate STZ 

摘      要：Backgrounds:Streptozotocin(STZ)-induced diabetic monkey is a wide used preclinical animal model for the investigation of diabetes such as islet transplantation and development of diabetic *** are serious side effects of this method,including nausea,emesis,weight loss,liver damage,renal failure,and metabolic *** order to reduce the side effects,diabetic monkeys were induced using clinicalgrade ***,clinical-grade STZ is not available in ***,we establised a method by using 100 mg/kg analytical-grade STZ to induce complete diabetes in cynomolgus monkey without generating adverse effects to liver and ***:Three cynomolgus monkeys were used in this study.100 mg/kg STZ dissolved in normal saline and infused through vein line in 5 minutes after indwelling catheter in the carotid artery and jugular *** the STZ administration,blood glucose levels were examined every 1 or 2 hours in the first 48 ***,blood glucose levels were examined twice per day during the first week after the STZ *** and C-peptide levels were measured by *** chemistry of hepatic and renal function tests were *** and glucagon expression in the islet of diabetic monkey and normal monkey were examined by immunohistochemistry ***:The stimulated C-peptide level(Intravenous glucose tolerance test)which is less than 0.5 ng/mL,the triphasic blood glucose response and the destroyed β cell suggested the complete induction of diabetes *** apparent adverse effects were observed including no signs of vomiting and toxicity after STZ ***:In summary,we established a safe and reproducible STZ-induced diabetic cynomolgus monkey model for islet transplantation which will be used to develop novel approaches for the treatment of diabetes.