Differences between karyotypically normal and abnormal human embryonic stem cells

作     者：Yang, S. Lin, G. Tan, Y. -Q. Deng, L. -Y. Yuan, D. Lu, G. -X. 

作者机构：Cent S Univ Inst Reprod & Stem Cell Engn Changsha Peoples R China Natl Engn Res Ctr Human Stem Cells Changsha Peoples R China 

出 版 物：《CELL PROLIFERATION》 (细胞增殖)

年 卷 期：2010年第43卷第3期

页      面：195-206页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：National Natural Science Foundation Major Program of China Hi-Tech Research and Development of China [2003AA205181, 2006AA02A102] National Basic Research Program of China [00CB 51010] 

主　　题：EMBRYONIC stem cells KARYOTYPES GENE expression CELL proliferation CANCER cells 

摘      要：Objectives: To compare different biological characteristics of human embryonic stem cells (HESCs) between those with normal and those with abnormal karyotype. Materials and methods: Culture-adapted HESCs (chHES-3) with abnormal karyotype were compared with karyotypically normal cells, with regard to pluripotency and differentiation capacity, ultrastructure, growth characteristics, gene expression profiles and signalling pathways. Results: We found a new abnormal karyotype of HESCs. We observed that chHES-3 cells with normal and abnormal karyotypes shared similarities in expression markers of pluripotency;however, karyotypically abnormal chHES-3 cells had a tendency for differentiation towards ectoderm lineages and were easily maintained in suboptimal culturing conditions. Abnormal chHES-3 cells displayed relatively mature cell organelles compared to normal cells, and karyotypically abnormal chHES-3 cells had increased survival and population growth. Genes related to cell proliferation and apoptosis were up-regulated, but genes associated with genetic instability (p53, Rb, BRCA1) were down-regulated in the karyotypically abnormal cells. Conclusion: Karyotypically abnormal chHES-3 cells had a more developed capacity for proliferation, resistance to apoptosis and less genetic stability compared to normal chHES-3 cells and may be an excellent model for studying and characterizing initial stages that determine transition of embryonic stem cells into cancer stem cells.