Magnetic Micromanipulation for In Vivo Measurement of Stiffness Heterogeneity and Anisotropy in the Mouse Mandibular Arch

作     者：Min Zhu Kaiwen Zhang Hirotaka Tao Sevan Hopyan Yu Sun 

作者机构：Department of Mechanical and Industrial EngineeringUniversity of TorontoCanada M5S 3G8 Program in Developmental and Stem Cell BiologyResearch InstituteThe Hospital for Sick ChildrenTorontoONCanada M5G 0A4 Department of Molecular GeneticsUniversity of TorontoCanada M5S 1A8 Division of Orthopaedic SurgeryThe Hospital for Sick Children and University of TorontoCanada M5G 1X8 Institute of Biomaterials and Biomedical EngineeringUniversity of TorontoCanada M5S 3G9 Department of Electrical and Computer EngineeringUniversity of TorontoCanada M5S 3G4 

出 版 物：《Research》 (研究（英文）)

年 卷 期：2020年第2020卷第1期

页      面：1409-1419页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This work was funded by the Canadian Institutes of Health Research(CIHR) Natural Sciences and Engineering Research Council of Canada(NSERC),Ontario Research Funds,and Medicine by Design–New Ideas Program 

主　　题：transition. stiffness anisotropic 

摘      要：The mechanical properties of tissues are pivotal for morphogenesis and disease *** approaches have enabled measurements of the spatial distributions of viscoelastic properties among embryonic and pathological model systems and facilitated the generation of important hypotheses such as durotaxis and tissue-scale phase *** likely are many unexpected aspects of embryo biomechanics we have yet to discover which will change our views of mechanisms that govern development and *** area in the blind spot of even the most recent approaches to measuring tissue stiffness is the potentially anisotropic nature of that ***,we report a magnetic micromanipulation device that generates a uniform magnetic field gradient within a large workspace and permits measurement of the variation of tissue stiffness along three orthogonal *** applying the device to the organ-stage mouse embryo,we identify spatially heterogenous and directionally anisotropic stiffness within the mandibular *** properties correspond to the domain of expression and the angular distribution of fibronectin and have potential implications for mechanisms that orient collective cell movements and shape tissues during *** of anisotropic properties extends the repertoire of current methods and will enable the generation and testing of hypotheses.