长臀脑垂体和血清中促性腺激素的生殖周期变化

作     者：周立斌 刘晓春 林浩然 叶卫 

作者机构：中山大学水生经济动物研究所和广东省水生经济动物良种繁育重点实验室广州510275 番禺国家级罗非鱼良种场广州番禺511453 

出 版 物：《动物学报》 (ACTA ZOOLOGICA SINICA)

年 卷 期：2003年第49卷第3期

页      面：399-403页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：国家自然科学基金资助项目 ( 39970 5 86 ) 广东海洋渔业局淡水鱼类繁育中心资助项目 

主　　题：长臀鮠 脑垂体 血清 促性腺激素 生殖周期变化 鱼类 

摘      要：Changes in the pituitary and serum gonadotropin levels of Cranoglanis bouderius were studied at different stages of ovarian development. Based on African catfish gonadotropin (cfGTH) and its anti-serum (Racf), heterologous radio-immunoassay (RIA) for the gonadotropin (GTH) of Cranoglanis bouderius was developed. The pituitary GTH contents at the late development stage were significantly higher than that at the mature, ovulation or regressed stages, while serum GTH levels at maturation and ovulation were significantly higher than that at late development and regressed stages. Injection of gonadotropin-releasing hormone analogue (GnRH-A) stimulated GTH secretion. Domperidone (DOM) had no effect on the basal serum GTH level. Injection of GnRH-A and DOM significantly enhanced the GTH levels stimulated by GnRH-A alone. These results indicate that GTH release in Cranoglanis bouderius is under the dual regulation of gonadotropin releasing hormone (GnRH) and dopamine (DA) from the hypothalamus, but DA can only inhibit GTH release induced by GnRH and not basal GTH release.