Elucidating the Origin of Heterogeneous Anomalous Diffusion in the Cytoplasm of Mammalian Cells

作     者：Adal Sabri Xinran Xu Diego Krapf Matthias Weiss 

作者机构：Experimental Physics I University of Bayreuth D-95440 Bayreuth Germany Department of Electrical and Computer Engineering Colorado State University Fort Collins Colorado 80523 USA School of Biomedical Engineering Colorado State University Fort Collins Colorado 80523 USA 

出 版 物：《Physical Review Letters》 (物理评论快报)

年 卷 期：2020年第125卷第5期

页      面：058101-058101页

核心收录：

学科分类：07[理学] 0702[理学-物理学] 

基　　金：Deutscher Akademischer Austauschdienst, DAAD, (57315749) Volkswagen Foundation, (92738) 

主　　题：Anomalous diffusion Cell processes & properties Fractional Brownian motion Intracellular transport Noise Cellular compartments 

摘      要：Diffusion of tracer particles in the cytoplasm of mammalian cells is often anomalous with a marked heterogeneity even within individual particle trajectories. Despite considerable efforts, the mechanisms behind these observations have remained largely elusive. To tackle this problem, we performed extensive single-particle tracking experiments on quantum dots in the cytoplasm of living mammalian cells at varying conditions. Analyses of the trajectories reveal a strong, microtubule-dependent subdiffusion with antipersistent increments and a substantial heterogeneity. Furthermore, particles stochastically switch between different mobility states, most likely due to transient associations with the cytoskeleton-shaken endoplasmic reticulum network. Comparison to simulations highlight that all experimental observations can be fully described by an intermittent fractional Brownian motion, alternating between two states of different mobility.