Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae ***--infection and melatonin supplementation

作     者：Xian Chen Pedro Laborda Yan Dong Fengquan Liu 

作者机构：Institute of Plant ProtectionJiangsu Key Laboratory for Food Quality and Safety–State Key Laboratory Cultivation Base of Ministry of Science and TechnologyJiangsu Academy of Agricultural SciencesNanjing 210014PR China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-productsZhejiang Academy of Agricultural SciencesHangzhou 310021PR China School of Life SciencesNantong UniversityNantong 226019PR China 

出 版 物：《Food Production, Processing and Nutrition》 (食物生产加工与营养（英文）)

年 卷 期：2020年第2卷第1期

页      面：207-216页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts(2010DS700124-KF2007) the National Natural Science Foundation of China(31571974) the Special Fund for Agroscientific Research in the Public Interest(201303015) the National Key R&D Program of China(2017YFD0200900) the Natural Science Foundation of Jiangsu Province of China(BK20170606) 

主　　题：Rice Xanthomonas oryzae pv.oryzae Melatonin qRT-PCR Reference genes 

摘      要：Exogenous melatonin(MT)was found to be an interesting tool for enhancing the resistance of rice to ***(Xoo)-caused bacterial blight(BB).However,the accurate comparison of the expression levels across samples was a challenging *** this work,the stability of 10 common used housekeeping genes under Xoo-infection and MT supplementation in rice was analyzed using quantitative real-time PCR(qRT-PCR),and algorithms geNorm,NormFinder and *** results indicated that most reference genes remained stable in Xoo-infected rice plants,while a number of reference genes were affected by MT *** all studied genes,the transcript levels of 18S(18S ribosomal RNA)and UBC(Ubiquitin-conjugating enzyme E2)remained unaltered by Xoo infection,while UBC and UBQ5(Ubiquitin 5)were the most stable genes when examining simultaneous Xoo-infection and MT supplementation,demonstrating that UBC is a suitable reference gene for qRT-PCR data normalization in rice under Xoo-infection and MT supplementation.