Screening for fragrance rice based on Badh2 gene

作     者：Siti Afiyatus Sholihah Elhah Nailul Khasna Nur Diniyah Suharti Dwi Listyorini 

作者机构：1Department of Biology Faculty of Mathematic and Natural Sciences Universitas Negeri Malang Jl. Semarang 5 Malang 65145 Indonesia 3Biotechnology Division Central Laboratory of Mineral and advanced Material Faculty of Mathematics and Natural Sciences Universitas Negeri Malang Indonesia Jl Semarang 5 Malang Indonesia 65145 2Department of Chemistry Faculty of Mathematic and Natural Sciences Universitas Negeri Malang Jl. Semarang 5 Malang 65145 Indonesia 4PUIPT Disruptive Learning Innovation Universitas Negeri Malang Jl Semarang 5 Malang Indonesia 65145 5Biotechnology Research Consortium 4in1Islamic Development Bank (IsDB) Program Indonesia 

出 版 物：《AIP Conference Proceedings》 

年 卷 期：2020年第2260卷第1期

学科分类：07[理学] 0702[理学-物理学] 

摘      要：Fragrance rice (Oryza sativa) recognized as an international crop commodity with high price. Betaine- aldehyde-dehydrogenase-2 (Badh2) is known as a key role in the accumulation of fragrant compound in rice, 2-acetyl-1- pyrroline (2AP). Nucleotide deletion of 8bp in exon 7 inactivates Badh2 causing in the increasing level of 2AP. The objective of this study was to determine among 20 Indonesian local rice varieties from Banten, Samarinda and Banyuwangi which genetically potential for producing fragrance rice based on Badh2 gene. Total DNA was isolated from leaves and Badh2 gene was amplified using PCR with specific primers. The evaluation based on amplified Badh2 fragments was carried out by electrophoresis in 1% agarose. Based on this study we suggest that Pandan Ungu from Samarinda and Blambangan A3 from Banyuwangi are the varieties which potential to produce fragrance rice.