Genetic relationship between local rice varieties based on MatK and rbcL genes

作     者：Nina Bunga Anggraini Annisa Sholihah Elhah Nailul Khasna Riri Wiyanti Retnaningtyas Suharti Dwi Listyorini 

作者机构：1Department of Biology Faculty of Mathematics and Natural Sciences Universitas Negeri Malang Jl Semarang Malang 65145 Indonesia 5Biotechnology Division Central Laboratory of Mineral and advanced MaterialFaculty of Mathematics and Natural Sciences Universitas Negeri Malang Jl Semarang Malang 65145 Indonesia 3Faculty of Biology Universitas Gadjah Mada Bulaksumur Yogyakarta 55281 Indonesia 4Cell and Molecular Biology Program Department of Biological Sciences University of Arkansas Fayetteville AR 72701 United States 2Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Negeri Malang Jl Semarang Malang 65145 Indonesia 6PUIPT Disruptive Learning Innovation Universitas Negeri Malang Jl Semarang Malang 65145 Indonesia 

出 版 物：《AIP Conference Proceedings》 

年 卷 期：2020年第2260卷第1期

学科分类：07[理学] 0702[理学-物理学] 

摘      要：Indonesia home of abundant indigenous and hybrid rice varieties. The phenomenon of phenotipic-plasticity allows varieties or even the same species to exhibit different phenotypic characteristics when planted in different locations. Thus, the identification based on phenotypic characters is needed to be supported by genetic data. The aim of this research was to unveil the relationship between local rice varieties provided from East Java, Banten, and Samarinda based on matK and rbcL genes. Total DNA from leaves were used as template for amplification of both genes. Phylogenetic trees were reconstructed using Neighbour-Joining (NJ) models in Kimura-2 parameters with 1000 bootstraps. The phylogenetic analysis using matK proved that rice varieties from East Java were closely related to rice varieties from Banten rather than to rice varieties from Samarinda. Meanwhile the same study using the rbcL gave inconsistent results