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作者机构:UNIV NACL MAR DEL PLATA INST INVEST BIOL RA-7600 MAR PLATA ARGENTINA
出 版 物:《JOURNAL OF PLANT PHYSIOLOGY》 (植物生理学杂志)
年 卷 期:1989年第134卷第2期
页 面:214-217页
核心收录:
学科分类:0710[理学-生物学] 071001[理学-植物学] 07[理学]
主 题:Triticum aestivum fructans sucrose sucrose phosphate synthase sucrose synthase CHI cycloheximide DP degree of polymerization FS fructosyl sucrose F 2 S fructosyl fructosyl sucrose FW fresh weight SPS sucrose phosphate synthase SS sucrose synthase TBA thiobarbituric acid
摘 要:The effect of carbohydrate supply on sucrose metabolism was studied in wheat leaf sections. Incubation with glucose, fructose or sucrose resulted in an increase of sucrose level followed by accumulation of fructans. Concomitantly, surcose phosphate synthase (SPS) activity increases continuously throughout the length of the experiment (48 hours) while sucrose synthase (SS) was unaffected for the first 30 hours. The de novo synthesis of SPS is suggested by Western blots and by inhibitiors of protein synthesis. The different temporal pattern sof SPS and SS activation indicate the distinct roles played by these enzymes in leaves.