Regulatory role of L-proline in fetal pig growth and intestinal epithelial cell proliferation

作     者：Jing Wang Bi'e Tan Jianjun Li Xiangfeng Kong Minjie Tan Guoyao Wu Jing Wang;Bi'e Tan;Jianjun Li;Xiangfeng Kong;Minjie Tan;Guoyao Wu

作者机构：Hunan International Joint Laboratory of Animal Intestinal Ecology and HealthLaboratory of Animal Nutrition and Human HealthCollege of Life SciencesHunan Normal UniversityChangsha 410081China College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Laboratory of Animal Nutritional Physiology and Metabolic ProcessKey Laboratory of Agro-ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Department of Animal ScienceTexas A&M UniversityCollege StationTX 77843USA 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2020年第6卷第4期

页      面：438-446页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by National Key R&D Program(2017YFD0500503) Postdoctoral Science Foundation(BX20180096) Hunan Province Science and Technology Projects(2017RS3059) Innovation Province Project(2019RS3021)in China Texas A&M AgriLife Research(H-8200)in USA 

主　　题：L-proline Polyamine metabolism Ornithine decarboxylase a-difluoromethylornithine Intestine Pig 

摘      要：L-proline(Pro)is a precursor of ornithine,which is converted into polyamines via ornithine decarboxylase(ODC).Polyamines plays a key role in the proliferation of intestinal epithelial *** study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in ***-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77%alanine(Ala,iso-nitrogenous control),1%Pro or 1%Pro+0.0167%α-difluoromethylornithine(DFMO)from d 15 to 70 of *** fetal body weight and number of fetuses per litter were determined,and the small and large intestines were obtained on d 70±1.78 of *** in vitro study was performed in intestinal porcine epithelial(IPEC-J2)cells cultured in Dulbecco’s modified Eagle medium-high glucose(DMEM-H)containing 0μmol/L Pro,400μmol/L Pro,or400μmol/L Pro+10 mmol/L DFMO for 4 *** results showed that maternal dietary supplementation with 1%Pro increased fetal weight;the protein and DNA concentrations of the fetal small intestine;and mRNA levels for potassium voltage-gated channel,shaker-related subfamily,member 1(Kv1.1)in the fetal small and large intestines(P0.05).Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells(P0.05).In comparison with the Pro group,the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine,as well as the concentrations of putrescine,spermidine and spermine in IPEC-J2 cells(P0.05).Meanwhile,the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation,whereas depletion of cellular polyamines with DFMO increased tumor protein p53(p53)mRNA levels(P0.05).Taken together,dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell