This paper aimed to explore the protective effects of picroside Ⅱ against the neuronal apoptosis and changes in morphology and structure that follow cerebral ischemic injury in rats.A focal cerebral ischemic model wa...
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This paper aimed to explore the protective effects of picroside Ⅱ against the neuronal apoptosis and changes in morphology and structure that follow cerebral ischemic injury in rats.A focal cerebral ischemic model was established by inserting a monofilament thread to achieve middle cerebral artery occlusion (MCA0) in 60 Wistar rats, and intraperitoneal injections of picroside Ⅱ (20 mg/kg) were *** neurobehavioral functions were evaluated with the modified neurological severity score (mNSS) *** cerebral infarct volumes were measured with tetrazolium chloride (TTC) *** morphology and ultrastructure of the cortical brain tissues were observed with hematoxylin-eosin staining and transmission electron microscopy, *** apoptotic cells were counted with terminal deoxynucleotidyl transferase dUTP nick-end labeling and flow cytometry, and pERK1/2 expression was determined by immunohistochemical *** results indicated that neurological behavioral malfunctions and cerebral infarcts were present in the MCA0 *** the model group, the damage to the structures of the neurons and the blood brain barrier (BBB) in the cortex was more severe, and the numbers of apoptotic cells, the early apoptotic ratio (EAR) and pERK1/2 expression were significantly increased in this group compared to the control group (P<0.05).In the treatment group,the neurological behavioral function and the morphology and ultrastructure of the neurons and the BBB were improved, and the cerebral infarct volume, the number of apoptotic cells, the EAR and pERK1/2 expression were significantly decreased compared to the model group (P<0.05).These results suggest that picroside Ⅱ reduced apoptosis and improved the morphology and ultrastructure of the neurons and the BBB and that these effects resulted in the recovery of the neurobehavioral function of rats with cerebral ischemia.
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