Psoriasis is an immune-mediated chronic skin disease caused by immune system mistakes and overproduction of new skin ***, plaque-like, scaly, painful and disfiguring skin lesions occur in the skin of psoriatic patient...
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Psoriasis is an immune-mediated chronic skin disease caused by immune system mistakes and overproduction of new skin ***, plaque-like, scaly, painful and disfiguring skin lesions occur in the skin of psoriatic patients [1].Imiquimod (IMQ) is a TLR7/8 ligand and potent immune activator, which can induce and exacerbate psoriasis [2].The aim of this work is to compare the permeability differences of three transdermal marker drugs on IMQ-induced psoriasis like mouse skin and normal *** C57/BL6 mouse was anesthetized using chloral hydrate and *** IMQ was applied on the skin for a few days to form the psoriatic *** psoriatic skin was confirmed using HE *** and psoriatic mouse skin was mounted on side by side transdermal diffusion cell (PermeGear, US) with the stratum corneum facing the donor *** widely used transdermal drug named Indomethacin (IM), idocaine (LD), and 5-fluorouracil (5-FU)with the log P of 4.25, 2.20 and-0.95 were selected as the model *** drug in isopropyl myristate was added in the donor cells while PBS (pH 7.4) was added in the acceptor *** system was maintained at 32 ℃ for 8 *** of 2 mL was withdrew and supplemented at predetermined *** centrifugation, the sample was measured using HPLC and the penetration flux was *** results of model establishment were shown in *** psoriatic skin showed desquamation, erythema and epidermis *** with the appearance of skin photos, the hyperplasia of basal and suprabasal keratinocytes expressed in psoriatic skin in HE staining *** in vitro penetration, the penetration flux of IM, LD and 5-FU through psoriatic skin was 2.06, 1.55 and 1.11 times higher to that of normal skin (Fig.2),which means higher speed penetration through psoriatic *** there is a proportional relation between the permeation enhancing effect and the value of log *** pathological membrane of psoriasis showed high
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