A high-performance liquid chromatography-DAD method was applied to the determination of vanillic acid,caffeic acid,ferulic acid,protocatechuic acid,paeoniflorin, baicalin and scutellarin in Qin-Lian-Si-Wu decoction an...
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A high-performance liquid chromatography-DAD method was applied to the determination of vanillic acid,caffeic acid,ferulic acid,protocatechuic acid,paeoniflorin, baicalin and scutellarin in Qin-Lian-Si-Wu decoction and other 5 crude *** eight compounds were analysed simultaneously with a Diamonsil C-18 column by gradient elution using 0.5%(v/v) acetic acid-acetonitrile as the mobile *** follow rate was 0.8mL/min, the temperate was 30℃,and detection was set at 230,260,280,310,323 *** linearity (r≥0.9995) of the 8 compounds was good in a relatively wide concertration rage,and the average recovery was 96.1～101.3%.The result indicated that the content of these eight compounds changed after decocting *** the compounds decreased significantly.
As the complexity of the chemical constitution of traditional Chinese medicines (TCMs),it is now a trend to establish a method of using multi-components analysis for the effective quality control of ***,the limited av...
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As the complexity of the chemical constitution of traditional Chinese medicines (TCMs),it is now a trend to establish a method of using multi-components analysis for the effective quality control of ***,the limited availability of multiple reference substances hinders the wide popularization of this method for the routine quality control of *** an easy-available single component which contained in the analyte as internal/external standard to determine multiple analogues should be an optional *** this paper,we select Rhubarb rhizome as an example,using emodin,an easy-available active component,as external standard to determine directly its own content in Rhubarb,meanwhile as internal standard to determine simultaneously the other six *** with the results obtained by external standard method,this alternative method was found in our laboratory to have no significant difference verified by the F-test(p = 95%,n = 6).However,on account of the weak robustness caused by some fluctuation of relative response factors from different laboratories,such a method can only serve as an expedient measure for solving the lack of chemical standards,and first relative response factors have to be determined before this method can be used without reference *** the corresponding chemical standards being available as well as being acceptable cost/effective,the external standard method for simultaneous determination of multiple components(SDMC) of TCMs will prevail.
Longdan Xiegan Decoction（LXD） is a formulated preparation composed of ten ingredient herbs,with iridoids as one group of the main bioactive *** this study,a rapid,simple and reliable method of simultaneous determina...
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Longdan Xiegan Decoction（LXD） is a formulated preparation composed of ten ingredient herbs,with iridoids as one group of the main bioactive *** this study,a rapid,simple and reliable method of simultaneous determination of four iridoid glycosides in LXD using high-performance liquid chromatography（HPLC） coupled with electrospray ionization mass spectrometry（MS） was first developed and validatedThe four iridoid glycosides references were isolated from LXD extract and purified using a preparative HPLC chromatography（the purities of all four analytes≥98%for assay purpose）.The sample preparation for quantification comprised of a simple ultrasonic extraction and the separation of four structurally similar iridoid glycosides was effected by HPLC(CAPCELL PAK C MGⅡcolumn,3μm,100 mm×2.0 mm) using an elution system of 10%methanol Satisfactory chromatographic separation of these analytes was obtained in less than three minutes and the concentrations of four iridoid glycosides in different batches of LXD and ingredient herbs were simultaneously determined by HPLC-MS/MS using a multiple reaction monitoring（MRM） *** method was validated with respect to the overall intra- and inter-day variation（RSD less than 8%） and the limits of quantification for the four iridoid glycosides were 35,20,37 and 33 ng/mL,respectively. The main result are shown in Figures 1-3 and tables 1-3.
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