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Isolation,purification,structural analysis and Immunostimulatory activity of water-soluble polysaccharides from the root of Glehnia littoralis

Isolation,purification,structural analysis and Immunostimula...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Du Bao xiang Rong Rong Shandong University of Traditional Chinese Medicine ShandongJinan250355China

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Metabonomics in the toxicity studies of traditional Chinese medicine

Metabonomics in the toxicity studies of traditional Chinese ...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Wang Xin Liu Siyan Pan Yixuan Zhang Mei School of Pharmacy Chengdu University of Traditional Chinese MedicineKey Laboratory of Standardization of Chinese Herbal Medicines of Ministry of EducationState Key Laboratory Breeding Base of Systematic ResearchDevelopment and Utilization of Chinese Medicine ResourcesChengdu 611137China

Traditional Chinese Medicine(TCM)has played a significant role through a developing progress of diseases for thousands years,and caught attentions all over the word *** the same time,the toxicity of TCM has come into notice as ***,investigating the toxicity of TCM has become essential and many methods have been *** decades,it is metabonomics that has been novelly used in the area of toxicity study of *** a branch of systems biology,metabonomics possesses significant advantages and potency in studying toxicity of *** this review,metabonomics was briefly talked;some examples about nephrotoxicity,hepatotoxicity,and cardiotoxicity were quoted;and also,some discussions and ideas were presented in the end.

关键词： metabonomics toxicity traditional Chinese medicine

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A comprehensive screening and identification of genistin metabolites in rat urine based on UHPLC-LTQ-Orbitrap mass spectrometer

A comprehensive screening and identification of genistin met...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Yaoyue Liang Chenxiao Wang Wenjing Zhao Tianyu Lou Jiayu Zhang Zhibin Wang School of Chinese Pharmacy Beijing University of Chinese MedicineBeijing 100029China Beijing Research Institution of Chinese Medicine Beijing University of Chinese MedicineBeijing 1000

Genistin,an isoflavone belonging to the phytoestrogen family,is mainly isolated from leguminous plants,has been reported to possess various therapeutic *** to the insufficient study on the in vivo metabolic pathways of genistin until now,in this paper,the genistin metabolites were investigated in rat urine by UHPLC-LTQ-Orbitrap mass spectrometer in both positive and negative ion *** diagnostic product ions(DPIs)and neutral loss fragments(NLFs)were proposed to efficiently identify and ascertain the major-to-trace genistin ***,the calculated Clog P values were utilized to distinguish the positional isomers with different retention ***,a total of 37 metabolites including prototype drug were positively or tentatively characterized based on the reference standards,accurate mass measurements,chromatographic retention behaviors,mass fragmentation pathways and corresponding calculated Clog P *** results demonstrated that genistin mainly underwent methylation,hydrogenation,hydroxylation,glucuronide conjugation,sulfate conjugation,acetylation and their composite reactions in vivo *** conclusion,our research revealed the genistein metabolites and metabolic pathways in vivo comprehensively.

关键词： genistin UHPLC-LTQ-Orbitrap mass spectrometer metabolic profiling

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Quality assessment of crude and processed Leonuri Fructus by chemical and color analysis combined with chemometric method

Quality assessment of crude and processed Leonuri Fructus by...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Shuiqing Li Xindan Liu Donghui Yan Xianmei Deng Shumei Wang Ying Zhang Jiang Meng Department of Traditional Chinese Medicine Guangdong Pharmaceutical University/Key Laboratory of Dig College of Pharmacy Jinan UniversityGuangzhou 510632China Zhaoqing Medical College Zhaoqing 526020China

Objective:Crude Leonuri Fructus(CLF),the fruits of the Leonurus japonicus Houtt.,and processed Leonuri Fructus(PLF)by stir-baking as the important Chinese herbal medicines,have been used in China and other Asian countries for thousands of *** objective of this research is to reveal the difference between CLF and ***:The sensory technologies of the colorimetry,sensitive and validated HPLC-ELSD and GC combined with flame ionization detector(GC-FID)were employed to discriminate crude Leonuri Fructus and its processed product *** color parameters of the samples were determined by colorimetric instrument ***,the content of stachydrine and six fatty acids were determined by HPLC and ***,analysis of variance(ANOVA),principal components analysis(PCA),hierarchical cluster analysis(HCA),and Kendall's correlation test were performed for data ***:The CLF and PLF were divided into two categories by PCA and HCA in terms of their component content and *** results distinctly demonstrated significant changes in color and the content of indicative components between CLF and ***:The study revealed that the HPLC,GC,and colorimetric method in combination with chemometric method could be used as comprehensive quality evaluation for CLF and PLF.

关键词： Color analysis Content determination Leonuri Fructus Hierarchical cluster analysis(HCA) Principal component analysis(PCA)

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Anintegrated strategy to qualitatively differentiate components of raw and processed Viticis Fructus based on NIR,HPLC and UPLC-MS analysis

Anintegrated strategy to qualitatively differentiate compone...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Jia-Yin Diao Fei Sun Yu Zhang Yue-Wei Ge Shu-Mei Wang School of Chinese Materia Medica Guangdong Pharmaceutical UniversityGuangzhouChina School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou China Key Laborato

Viticis Fructus(VF)is a traditional Chinese herbal drug processed by various methods to achieve different clinical *** treatment potentially alters chemical composition,which may impact on effectiveness and *** order to interpret the constituent discrepancies of raw versus processed(stir-fried)VF,a multivariatedetection method(NIR,HPLC,and UPLC-MS)based on metabonomics and chemometricswas ***,synergy interval partial least squares(siPLS)and partial least squares-discriminated analysis(PLS-DA)was employed to screen the distinctive wavebands(4319-5459cm-1)based on preprocessed NIR ***,high-performance liquid chromatography(HPLC)with principal component analysis(PCA)was performed to characterize the ***,a total of 49 compounds were identified by ultra-performance liquid chromatography with mass spectrometry(UPLC-MS),among which 42 compounds were eventually characterized to have the significant change during processing via the semi-quantitative volcano plot ***,based on the PCA-PLS-DA analysis,16 compounds were chosen as characteristic markers which could be in close correlation with the discriminatory NIR ***,all of these characterization techniques effectively discriminated raw and processed products of *** general,our work provides an integrated way of classifying VF and a strategy to explore discriminatory chemical markers for other traditional Chinese herbs,thus ensuring safety and efficacy for consumers.

关键词： Viticis Fructus multivariate statistical analysis quality control Introduction

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Study on tanshinone Ⅰ and tanshinone ⅡA metabolites in vivo in rats

Study on tanshinone Ⅰ and tanshinone ⅡA metabolites in viv...

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中华中医药学会中药分析分会第十一次学术交流会

作者： ZHAO Wen-jing LIANG Yao-yue WANG Chen-xiao WANG Zi-jian ZHANG Jia-yu WANG Zhi-bin School of Chinese Pharmacy Beijing University of Chinese MedicineBeijing 100102China Beijing Institution of Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China

An efficient method of ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap(UHPLC-LTQ-Orbitrap)mass spectrometer was established to elucidate the in vivo metabolic process of tanshinone Ⅰ and tanshinone ⅡA in *** and plasma samples were collected after oral *** the process of biological sample solid phase extraction,the gradient elution was performed using Waters ACQUITY BEH C18 column(2.1 mm×100 mm,1.7 μm,Waters Corporation,Milford,MA,USA)with 0.1%formic acid(A)-acetonitrile(B)solution as the mobile *** plasma,urine and the blank samples were detected by ESI-LTQ-Orbitrap equipped with an ESI ion source in positive ion *** the basis of the accurate mass measurements,multiple mass spectra and comparison of data with published literature,a total of 26 metabolites were tentatively identified and *** them,7 metabolites were derived from tanshinone Ⅰ through metabolic pathways of glucuronidation,hydroxylation,hydrogenation,demethylation,methylation,sulfonation,and their composite reactions.19 metabolites were elicited by tanshinone ⅡA through metabolic pathways of hydroxylation,hydrogenation,methylation,sulfonation,glucuronidation,glycosylation,and their complicated *** results comprehensively clarified the metabolism of tanshinone Ⅰ and tanshinoneⅡ A in rats using UHPLC-LTQ-Orbitrap mass spectrometer, which provided material basis for the further research in terms of pharmacodynamics, toxicology, and secondarydevelopment of Chinese medicine.

关键词： tanshinone Ⅰ tanshinone ⅡA UHPLC-LTQ-Orbitrap metabolites

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Chemical constituents from the Moutan Cortex charcoal and their potential coagulation activities

Chemical constituents from the Moutan Cortex charcoal and th...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Xiaolu Yang Xingyang Xue Yan Lin Qiyun Huang Maoyan Mo Shumei Wang Jiang Meng Department of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006Chin Guangzhou Medical University Cancer Hospital and Institute Guangzhou 510006China Department of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006Chin

The chemical constituents of Cortex Moutan charcoal were studied in depth,and their coagulation activity was *** chemical constituents were isolated by polyamide,silica gel and Sephadex G-Sepharose chromatography purification,and their structures were identified by NMR spectroscopy and other analyses.A total of 10 compounds were obtained and identified as follows:3-(2-furan)-1-(2-hydroxy-4-methoxy-phenyl)-2-propylene ketone(1),2-(2,5-hydroxy-4-methyl phenyl)propionate ethyl ester(2),methyl tetradec-5-enoate(3),1,4-diethylcyclohexane(4),catechol(5),methyl-4-hydroxybenzoate(6),3-hydroxy-2-methyl-4-pyrone(7),3,8-dihydroxy-2-metyl-chromone(8),3β-hydroxy-olean-12-en(9),1-monolinolein(10).Among them,compounds 1 and 2 were new natural products,and 3-10 were isolated from the Cortex Moutan charcoal for the first *** potential coagulation activities of compounds were evaluated,and compounds 2,9 and 10 had strong hemostatic *** compound 1 and 8 could significantly activate blood circulation.

关键词： Moutan Cortex charcoal Chemical constituents Coagulation activities

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Comparing pharmacokinetic study of Ixerin Z and 11,13α-dihydroixerin Z in rats after intravenous administration of the monomers and Kudiezi injection by UHPLC-MS/MS

Comparing pharmacokinetic study of Ixerin Z and 11,13α-dihy...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Dong Lu-yan Cai Wei Zhang Jia-yu Yao Hong Zhang Xiu-ping Liu Ying Lu Jian-qiu School of Chinese Pharmacy Beijing University of Chinese MedicineBeijing 100102China Research Institute of Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China Library Beijing University of Chinese MedicineChaoyang DistrictBeijing 100029China

An effective method based on ultra-high-performance liquid chromatography-tandem mass spectrometry was established for the simultaneous determination of Ixerin Z and 11,13α-dihydroixerin Z in rat *** was used as the internal standard(IS).Chromatographic separation was performed on an ethylene bridged hybrid Ci8 column using a gradient elution at a flow rate of 0.40 mL/*** detection was achieved on negative ion electrospray ionization in multiple-reaction monitoring *** calibration curves for the two analytes showed good linearity(R2>0.9974),and the lower limit of quantitation was 0.120 ng/mL and 0.150 ng/mL in rat plasma,*** intra-and inter-day precision and accuracy of IZ and DDIZ were within acceptable *** mean recoveries ranged from 75.1%to 91.3%.This validated method was the first to be successfully used in the pharmacokinetic study in rats following an intravenous administration of the two monomers and Kudiezi injection(KDZI).The results indicated that the pharmacokinetic behaviors were obviously different after the administration of KDZI as compared with administration of monomer *** suggests that the component-component interaction can change the pharmacokinetic behavior of component of traditional Chinese medicine.

关键词： Ixerin Z 1,13α-dihydroixerin Z UHPLC-MS/MS Pharmacokinetics Kudiezi injection

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Therapeutic Effects of Yinchenhao Decoction on Alpha-naphthylisothiocyanate Induced Intrahepatic Cholestasis in Rats

Therapeutic Effects of Yinchenhao Decoction on Alpha-naphthy...

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中华中医药学会中药分析分会第十一次学术交流会

作者： Yue Ding Ya-Xiong Yi Tong Zhang School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghaiChina201203 Experiment Center for Teaching and Learning Shanghai University of Traditional Chinese MedicineShan

Yinchenhao Decoction(YCHD),a famous traditional Chinese formula,has been used for treating cholestasis for thousands of *** cholagogic effect has been widely reported,but its pharmacodynamic material and underlying therapeutic mechanism remain *** using UHPLC-Q/TOF,eleven original active components and eight phase Ⅱ metabolites were detected in rats after oral administration of YCHD,including three new phase Ⅱ *** it indicated that phase Ⅱ metabolism was one of the major metabolic pathway for most active components in YCHD,which was similar to the metabolism process of *** arouses our curiosity that whether the metabolism process of YCHD has any relationship with its cholagogic *** a new method for simultaneous quantitation of 8 active components and 4 phase Ⅱmetabolites of rhein,emodin,genipin,and capillarisin has been developed and applied for their pharmacokinetic study in both normal and alpha-naphthylisothiocyanate(ANIT)-induced intrahepatic cholestasis *** pharmacokinetic behaviors of most components of YCHD were inhibited,which was hypothesized to be related to different levels of metabolic enzymes and transporters in rat *** dynamic changes of intrahepatic enzyme expression in cholestasis and YCHD treated rats have been monitored by an UHPLC-MS/MS *** results showed expression levels of UGT1A1,OATP1A4,MRP2 were significantly inhibited in cholestasis rats,which would account for reducing the drug absorption and the metabolic process of YCHD in cholestatic rats.A high dose(12 g/kg)of YCHD remarkably increased the expression of UGT1A1,bile salt export pump,MRP2,OATP1A4 in cholestasis rats presented it exhibited the greatest therapeutic effect on *** the metabolic process of bilirubin in vivo,the choleretic effect of YCHD is proven to be related to its regulatory action on expression of metabolic enzymes and transporters in cholestatic liver.

关键词： Yinchenhao Decoction phase Ⅱ metabolism pharmacokinetics cholestasis bilirubin metabolism enzyme metabolic transporter Introduction

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