Yellow camellia is Camellia nitidissima chi.,in the fanmily of Camellia,It is an inexpensive traditional Chinese medicine commonly used for the treatment of liver diseases,faucitis,diarrhea,edema,hypertension,tumor,an...
详细信息
Yellow camellia is Camellia nitidissima chi.,in the fanmily of Camellia,It is an inexpensive traditional Chinese medicine commonly used for the treatment of liver diseases,faucitis,diarrhea,edema,hypertension,tumor,and a few studies have indicated that Camellia nitidissima *** the effects of heat-clearing and detoxication,strangury-relieving,anti-cancer, inhibition of tumor growing,reducing blood lipid,preventing atherosclerosis,increasing the immune function,and drain *** the current study,raw Camellia nitidissima *** was extracted with 70%ethanol,extracte the crude ethanol extraction with 40-60%petroleum ether,ethyl acetate and 1-Butanol individually and get 5 *** test cytotoxicity effect with MTT ***,MTT stock solution(5 mg/ml) was diluted to 1 mg/ml in culture medium as a working MTT *** treated cells were added with working MTT solution(50 ul/well) to analyze cell *** 3 h incubation,the culture medium was *** formazan was dissolved with DMSO,and then the cell viability was measured by using microplate reader at absorbance 570 nm and 650 nm as a ***%inhibition and IC50 were calculated Conclusion F5(water fraction) can not determine the IC50,maybe need higher *** on the lower IC50 of 183.959 and lower concentration,F4(1-butanol fraction) would be the most effective fraction for the further anti-cancer research about *** Chi,
暂无评论