Yellow camellia is Camellia nitidissima chi.,in the fanmily of Camellia,It is an inexpensive traditional Chinese medicine commonly used for the treatment of liver diseases,faucitis,diarrhea,edema,hypertension,tumor,an...
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Yellow camellia is Camellia nitidissima chi.,in the fanmily of Camellia,It is an inexpensive traditional Chinese medicine commonly used for the treatment of liver diseases,faucitis,diarrhea,edema,hypertension,tumor,and a few studies have indicated that Camellia nitidissima *** the effects of heat-clearing and detoxication,strangury-relieving,anti-cancer, inhibition of tumor growing,reducing blood lipid,preventing atherosclerosis,increasing the immune function,and drain *** the current study,raw Camellia nitidissima *** was extracted with 70%ethanol,extracte the crude ethanol extraction with 40-60%petroleum ether,ethyl acetate and 1-Butanol individually and get 5 *** test cytotoxicity effect with MTT ***,MTT stock solution(5 mg/ml) was diluted to 1 mg/ml in culture medium as a working MTT *** treated cells were added with working MTT solution(50 ul/well) to analyze cell *** 3 h incubation,the culture medium was *** formazan was dissolved with DMSO,and then the cell viability was measured by using microplate reader at absorbance 570 nm and 650 nm as a ***%inhibition and IC50 were calculated Conclusion F5(water fraction) can not determine the IC50,maybe need higher *** on the lower IC50 of 183.959 and lower concentration,F4(1-butanol fraction) would be the most effective fraction for the further anti-cancer research about *** Chi,
The genus Leonurus has long been recognized as a natural group,but its interspecific relationship has not yet been studied in the light of sequence *** ITS regions and matK sequences of all subgenera of Leonurus in Ch...
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The genus Leonurus has long been recognized as a natural group,but its interspecific relationship has not yet been studied in the light of sequence *** ITS regions and matK sequences of all subgenera of Leonurus in China were amplified,sequenced and *** generated by maximum parsimony and neighbor-joining methods and division of the genus into two major *** phylogenetic results indicated that *** has the very close phylogenetic relationship with *** and supported the notion that *** acts as the bridge between *** and *** to analysis of the information given by ITS and matK sequences,we as well suggest that ITS sequences would be more suitable to serve as markers for authentication of Herba Leonuri than matK does.
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