Human embryonic stem cells(hESCs),derived from the inner cell mass of human blastocysts,are considered to be the most pluripotent of all stem *** feeder-dependent and feeder-free culture systems are applied to maintai...
Human embryonic stem cells(hESCs),derived from the inner cell mass of human blastocysts,are considered to be the most pluripotent of all stem *** feeder-dependent and feeder-free culture systems are applied to maintain self-renewal ***,hESCs of different culture systems showed different morphology,there might be different transcriptional and functional differences between these *** test this hypothesis,we first derived three hESCs lines under the culture system of human foreskin fibroblasts (HFF) combined with mTESR1,and the stem cell characteristics were *** then,we conducted a large-scale transcriptional profiling of IncRNAs and mRNAs in these three pairs of hESCs cultured under feeder-dependent and feeder-free culture systems using microarray technology.
Objective: Current studies showed that the clinical pregnancy rate and implantation rate of blastocysts formed in the fresh embryo transfer cycle and transferred on day 5 (D5) were higher than the rates on day 6 (D6)....
Objective: Current studies showed that the clinical pregnancy rate and implantation rate of blastocysts formed in the fresh embryo transfer cycle and transferred on day 5 (D5) were higher than the rates on day 6 (D6).However,the pregnancy outcomes of D5 and D6 blastocyst transfers in different studies using frozen-thawed cycles reached different *** relevant studies only performed simple statistical analyses on the clinical outcomes of frozen-thawed *** this study,We analyzed blastocyst quality and pregnancy outcomes by taking into account blastocyst chromosomes euploidy to provide a more in-depth investigation into the differences in the development potential of embryos frozen-thawed on different developmental stages (frozen-thawed day 5 (D5) and day 6 (D6) cycles) and their relationship with clinical pregnancy outcomes.
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